Literature DB >> 849267

Interaction of difluoro-oxaloacetate with aspartate transaminase.

P A Briley, R Eisenthal, R Harrison, G D Smith.   

Abstract

Diffluoro-oxaloacetate behaves as a competitive inhibitor of 2-oxoglutarate and as an uncompetitive inhibitor with respect to aspartate in steady-state kinetic experiments with cytoplasmic aspartate transaminase. In the presence of high concentrations of aspartate transaminase, difluoro-oxaloacetate is slowly transaminated to difluoro-aspartate, suggesting its use as a kinetic probe to study the reactions of the aminic form of the enzyme.

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Year:  1977        PMID： 849267      PMCID： PMC1164515          DOI： 10.1042/bj1610383

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  8 in total

1.  The complete amino acid sequence of cytoplasmic aspartate aminotransferase from pig heart.

Authors:  Y A. Ovchinnikov; C A. Egorov; N A. Aldanova; M Y. Feigina; V M. Lipkin; N G. Abdulaev; E V. Grishin; A P. Kiselev; N N. Modyanov; A E. Braunstein; O L. Polyanovsky; V V. Nosikov
Journal:  FEBS Lett       Date:  1973-01-01       Impact factor: 4.124

2.  Glutamic aspartic transaminase. I. Assay, purification, and general properties.

Authors:  W T JENKINS; D A YPHANTIS; I W SIZER
Journal:  J Biol Chem       Date:  1959-01       Impact factor: 5.157

3.  Interaction between beta-fluoro-oxaloacetate and glutamate-aspartate transaminase of heart mitochondria.

Authors:  E KUN; D W FANSHIER; D R GRASSETTI
Journal:  J Biol Chem       Date:  1960-02       Impact factor: 5.157

4.  The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters.

Authors:  R Eisenthal; A Cornish-Bowden
Journal:  Biochem J       Date:  1974-06       Impact factor: 3.857

5.  The molecular weight and other properties of aspartate aminotransferase from pig heart muscle.

Authors:  B E Banks; S Doonan; A J Lawrence; C A Vernon
Journal:  Eur J Biochem       Date:  1968-09-24

6.  Use of multivariate non-linear regression analysis in fitting enzyme kinetic models. An empirical study of the inhibition of aspartate aminotransferase by dicarboxylic acid substrate analogues.

Authors:  K N Haarhoff
Journal:  J Theor Biol       Date:  1969-01       Impact factor: 2.691

Review 7.  Structure and catalytic role of the functional groups of aspartate aminotransferase.

Authors:  P Fasella; C Turano
Journal:  Vitam Horm       Date:  1970       Impact factor: 3.421

8.  On the formation of S-(alpha,beta-dicarboxyethyl) derivatives of glutamic-aspartic aminotransferase.

Authors:  C Turano; A Giartosio; F Riva; P Fasella
Journal:  Biochem Biophys Res Commun       Date:  1964-06-15       Impact factor: 3.575
  8 in total
  3 in total

1.  Determination of half-reaction equilibrium in a ping-pong enzyme mechanism.

Authors:  G D Smith; R Harrison; R Eisenthal
Journal:  Neurochem Res       Date:  1996-09       Impact factor: 3.996

2.  [19F]fluorine nuclear-magnetic-resonance study of the interaction of difluoro-oxaloacetate with aspartate transaminase.

Authors:  P A Briley; R Eisenthal; R Harrison; G D Smith
Journal:  Biochem J       Date:  1977-05-01       Impact factor: 3.857

3.  Reaction of the aminic form of aspartate transaminase with difluoro-oxaloacetate.

Authors:  P A Briley; R Eisenthal; R Harrison; G D Smith
Journal:  Biochem J       Date:  1977-10-01       Impact factor: 3.857
  3 in total

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