Literature DB >> 8490015

Sequence-specific cleavage of DNA via nucleophilic attack of hydrogen peroxide, assisted by Flp recombinase.

A S Kimball1, J Lee, M Jayaram, T D Tullius.   

Abstract

Hydrogen peroxide is capable of effecting the cleavage of a specific phosphodiester bond in DNA, when used in concert with the recombinase enzyme Flp from Saccharomyces cerevisiae. This cleavage is not caused by oxidative damage of the DNA backbone but instead is the result of nucleophilic attack by peroxide. A single phosphorus-oxygen bond is broken in the reaction. Cleavage of DNA by peroxide also occurs with an inactive mutant of Flp in which the active site nucleophile tyrosine has been replaced by phenylalanine. Besides providing information on the mechanism of strand cleavage by Flp, these results may contribute to the development of new synthetic DNA cleavage reagents that act by hydrolytic and not radical chemistry.

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Year:  1993        PMID： 8490015     DOI： 10.1021/bi00069a002

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  10 in total

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Review 2.  The partitioning and copy number control systems of the selfish yeast plasmid: an optimized molecular design for stable persistence in host cells.

Authors:  Yen-Ting Liu; Saumitra Sau; Chien-Hui Ma; Aashiq H Kachroo; Paul A Rowley; Keng-Ming Chang; Hsiu-Fang Fan; Makkuni Jayaram
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3.  Identification of a potential general acid/base in the reversible phosphoryl transfer reactions catalyzed by tyrosine recombinases: Flp H305.

Authors:  Katrine L Whiteson; Yu Chen; Neeraj Chopra; Amy C Raymond; Phoebe A Rice
Journal:  Chem Biol       Date:  2007-02

4.  Double-stranded cleavage of pBR322 by a diiron complex via a "hydrolytic" mechanism.

Authors:  L M Schnaith; R S Hanson; L Que
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-18       Impact factor: 11.205

5.  Active site electrostatics protect genome integrity by blocking abortive hydrolysis during DNA recombination.

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6.  Mechanism of active site exclusion in a site-specific recombinase: role of the DNA substrate in conferring half-of-the-sites activity.

Authors:  J Lee; T Tonozuka; M Jayaram
Journal:  Genes Dev       Date:  1997-11-15       Impact factor: 11.361

7.  Unveiling hidden catalytic contributions of the conserved His/Trp-III in tyrosine recombinases: assembly of a novel active site in Flp recombinase harboring alanine at this position.

Authors:  Chien-Hui Ma; Agnieszka Kwiatek; Swetha Bolusani; Yuri Voziyanov; Makkuni Jayaram
Journal:  J Mol Biol       Date:  2007-02-20       Impact factor: 5.469

8.  Lambda integrase cleaves DNA in cis.

Authors:  S E Nunes-Düby; R S Tirumalai; L Dorgai; E Yagil; R A Weisberg; A Landy
Journal:  EMBO J       Date:  1994-09-15       Impact factor: 11.598

9.  Stereospecific suppression of active site mutants by methylphosphonate substituted substrates reveals the stereochemical course of site-specific DNA recombination.

Authors:  Paul A Rowley; Aashiq H Kachroo; Chien-Hui Ma; Anna D Maciaszek; Piotr Guga; Makkuni Jayaram
Journal:  Nucleic Acids Res       Date:  2015-05-20       Impact factor: 16.971

10.  Magnetic field alignment of stable proton-conducting channels in an electrolyte membrane.

Authors:  Xin Liu; Yi Li; Jiandang Xue; Weikang Zhu; Junfeng Zhang; Yan Yin; Yanzhou Qin; Kui Jiao; Qing Du; Bowen Cheng; Xupin Zhuang; Jianxin Li; Michael D Guiver
Journal:  Nat Commun       Date:  2019-02-19       Impact factor: 14.919
  10 in total

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