Literature DB >> 8487311

Purification and crystallization of human cyclin-dependent kinase 2.

J Rosenblatt1, H De Bondt, J Jancarik, D O Morgan, S H Kim.   

Abstract

The major transitions of the eukaryotic cell cycle are triggered by cyclin-dependent protein kinases. We report the purification and crystallization of the catalytic subunit of human cyclin-dependent kinase 2 (CDK2), which has been implicated in the control of the G1/S transition. CDK2 was purified in large quantities from insect cells infected with a recombinant baculovirus, and crystals of the protein were prepared and subjected to preliminary X-ray diffraction analysis.

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Year:  1993        PMID： 8487311     DOI： 10.1006/jmbi.1993.1248

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  13 in total

1.  Crystal structure of a gamma-herpesvirus cyclin-cdk complex.

Authors:  G L Card; P Knowles; H Laman; N Jones; N Q McDonald
Journal:  EMBO J       Date:  2000-06-15       Impact factor: 11.598

2.  A human primary T-lymphocyte-derived human immunodeficiency virus type 1 Tat-associated kinase phosphorylates the C-terminal domain of RNA polymerase II and induces CAK activity.

Authors:  S Nekhai; R R Shukla; A Kumar
Journal:  J Virol       Date:  1997-10       Impact factor: 5.103

3.  Structural studies of p21Waf1/Cip1/Sdi1 in the free and Cdk2-bound state: conformational disorder mediates binding diversity.

Authors:  R W Kriwacki; L Hengst; L Tennant; S I Reed; P E Wright
Journal:  Proc Natl Acad Sci U S A       Date:  1996-10-15       Impact factor: 11.205

4.  Structural basis for specificity and potency of a flavonoid inhibitor of human CDK2, a cell cycle kinase.

Authors:  W F De Azevedo; H J Mueller-Dieckmann; U Schulze-Gahmen; P J Worland; E Sausville; S H Kim
Journal:  Proc Natl Acad Sci U S A       Date:  1996-04-02       Impact factor: 11.205

5.  Identification of cylin-dependent kinase 1 inhibitors of a new chemical type by structure-based design and database searching.

Authors:  P Furet; T Meyer; P Mittl; H Fretz
Journal:  J Comput Aided Mol Des       Date:  2001-05       Impact factor: 3.686

6.  Cyclin-dependent kinase 9 (Cdk9) of fission yeast is activated by the CDK-activating kinase Csk1, overlaps functionally with the TFIIH-associated kinase Mcs6, and associates with the mRNA cap methyltransferase Pcm1 in vivo.

Authors:  Yi Pei; Hongyan Du; Juliet Singer; Courtney Stamour; Selena Granitto; Stewart Shuman; Robert P Fisher
Journal:  Mol Cell Biol       Date:  2006-02       Impact factor: 4.272

7.  Reciprocal activation by cyclin-dependent kinases 2 and 7 is directed by substrate specificity determinants outside the T loop.

Authors:  S Garrett; W A Barton; R Knights; P Jin; D O Morgan; R P Fisher
Journal:  Mol Cell Biol       Date:  2001-01       Impact factor: 4.272

8.  A novel inhibitor of cyclin-Cdk activity detected in transforming growth factor beta-arrested epithelial cells.

Authors:  J M Slingerland; L Hengst; C H Pan; D Alexander; M R Stampfer; S I Reed
Journal:  Mol Cell Biol       Date:  1994-06       Impact factor: 4.272

9.  Recombinant production of enzymatically active male contraceptive drug target hTSSK2 - Localization of the TSKS domain phosphorylated by TSSK2.

Authors:  Jagathpala Shetty; Rondedrick Sinville; Igor A Shumilin; Wladek Minor; Jianhai Zhang; Jon E Hawkinson; Gunda I Georg; Charles J Flickinger; John C Herr
Journal:  Protein Expr Purif       Date:  2016-01-14       Impact factor: 1.650

10.  Effects of phosphorylation by CAK on cyclin binding by CDC2 and CDK2.

Authors:  D Desai; H C Wessling; R P Fisher; D O Morgan
Journal:  Mol Cell Biol       Date:  1995-01       Impact factor: 4.272
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