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Literature DB >> 8486076

Detection of minimal amounts of DNA by electron microscopy using simplified spreading procedures.

Abstract

Electron microscopic examination of nucleic acids requires the use of special spreading techniques. The classical method was developed by Kleinschmidt and Zahn in 1959. Modifications of this method increased sensitivity to allow detection of a total amount of about 1 x 10(-3) micrograms of single-stranded DNA and 2 x 10(-5) micrograms of double-stranded DNA. Here we describe two rapid and simple procedures increasing sensitivity by 1-2 orders of magnitude to visualize at least 1 x 10(-5) micrograms of single- and/or double-stranded DNA.

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Year: 1993 PMID： 8486076 DOI： 10.1007/bf00352398

Source DB: PubMed Journal: Chromosoma ISSN： 0009-5915 Impact factor: 4.316

12 in total

Detection of minimal amounts of DNA by electron microscopy using simplified spreading procedures.

1. Coding sequence and flanking regions of the mouse vimentin gene.

2. Transcription of spacer sequences in genes coding for ribosomal RNA in Xenopus cells.

3. Length measurements of RNA synthesized in vitro by Escherichia coli RNA polymerase.

4. Simplified quantitative electron microscopy of biopolymers.

5. Partial denaturation of thymine- and 5-bromouracil-containing lambda DNA in alkali.

6. Electron-microscopic visualization of deletion mutations.

7. Nucleotide sequence of bacteriophage fd DNA.

8. Isolation and characterization of lambdadargECBH transducing phages and heteroduplex analysis of the argECBH cluster.

9. Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy.

10. Vimentin cDNA clones covering the complete intermediate-filament protein are found in an EHS tumor cDNA library.

1. Changes in chromosomal ultrastructure during the cell cycle.

2. Localization of hepatitis B virus core protein and viral DNA at the nuclear membrane.

3. Diheteroduplex formation using gold labeled single-stranded PCR fragments and its application in electron microscopy.

4. Hepatitis B virus genome is organized into nucleosomes in the nucleus of the infected cell.

5. Cross-linking of DNA through HMGA1 suggests a DNA scaffold.