Literature DB >> 84842

Reduction of the non-specific binding of DNA to gamma-globulin in Farr radioimmunoassay by addition of dextran sulfate and calcium chloride.

A Wakizaka, E Okuhara.   

Abstract

The effect of non-specific binding caused by the interaction between gamma-globulin and denatured DNA was markedly reduced by addition of dextran sulfate or CaCl2 at alkaline pH. This method was shown to be applicable in the detection of anti-DNA antibodies in sera from cases of human systemic lupus erythematosus.

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Year:  1979        PMID： 84842     DOI： 10.1016/0022-1759(79)90047-4

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  1 in total

1.  Antigenic structure of DNA: relatively high inhibitory activity of di- and trideoxyribonucleotide derived from DNase digest on the interaction of thermally denatured DNA with systemic lupus erythematosus sera.

Authors:  M Kashimura; A Wakizaka; K Kurosaka; E Okuhara; T Akihama; A B Miura; A Shibata
Journal:  Clin Exp Immunol       Date:  1981-02       Impact factor: 4.330
  1 in total

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