Literature DB >> 8481539

Antisense DNA oligonucleotides. I: The use of ionspray tandem mass spectrometry for the sequence verification of methylphosphonate oligodeoxyribonucleotides.

T R Baker1, T Keough, R L Dobson, T A Riley, J A Hasselfield, P E Hesselberth.   

Abstract

The sequences of synthetically prepared methylphosphonate oligodeoxyribonucleotides have been verified using ionspray tandem mass spectrometry with sample introduction via flow injection. The technique involves the use of product-ion scans from multiply protonated (4+ and 5+) precursors. Among the ions detected are several series of fragments of different charge states that indicate the base sequence of the intact molecule. Oligomers as large as 18 bases have been successfully characterized.

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Year:  1993        PMID： 8481539     DOI： 10.1002/rcm.1290070305

Source DB:  PubMed          Journal:  Rapid Commun Mass Spectrom        ISSN： 0951-4198            Impact factor:   2.419


  4 in total

Review 1.  Mass spectrometry of nucleotides and oligonucleotides.

Authors:  P Miketova; K H Schram
Journal:  Mol Biotechnol       Date:  1997-12       Impact factor: 2.695

2.  Dissociation energies of deoxyribose nucleotide dimer anions measured using blackbody infrared radiative dissociation.

Authors:  E F Strittmatter; P D Schnier; J S Klassen; E R Williams
Journal:  J Am Soc Mass Spectrom       Date:  1999-11       Impact factor: 3.109

3.  A procedure for selective DNA alkylation and detection by mass spectrometry.

Authors:  I G Gut; S Beck
Journal:  Nucleic Acids Res       Date:  1995-04-25       Impact factor: 16.971

4.  Synthesis and thermodynamics of oligonucleotides containing chirally pure R(P) methylphosphonate linkages.

Authors:  M A Reynolds; R I Hogrefe; J A Jaeger; D A Schwartz; T A Riley; W B Marvin; W J Daily; M M Vaghefi; T A Beck; S K Knowles; R E Klem; L J Arnold
Journal:  Nucleic Acids Res       Date:  1996-11-15       Impact factor: 16.971
  4 in total

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