Literature DB >> 8476297

Expression and secretion of Bacillus amyloliquefaciens alpha-amylase by using the yeast pheromone alpha-factor promoter and leader sequence in Saccharomyces cerevisiae.

V J Southgate1, A J Steyn, I S Pretorius, H J Van Vuuren.   

Abstract

Replacement of the regulatory and secretory signals of the alpha-amylase gene (AMY) from Bacillus amylolique-faciens with the complete yeast pheromone alpha-factor prepro region (MF alpha 1p) resulted in increased levels of extracellular alpha-amylase production in Saccharomyces cerevisiae. However, the removal of the (Glu-Ala)2 peptide from the MF alpha 1 spacer region (Lys-Arg-Glu-Ala-Glu-Ala) yielded decreased levels of extracellular alpha-amylase.

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Year:  1993        PMID： 8476297      PMCID： PMC202271          DOI： 10.1128/aem.59.4.1253-1258.1993

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  45 in total

1.  Expression and secretion vectors for yeast.

Authors:  G A Bitter; K M Egan; R A Koski; M O Jones; S G Elliott; J C Giffin
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

2.  Multiple amylase genes in two strains of Bacillus stearothermophilus.

Authors:  S Sen; P Oriel
Journal:  Gene       Date:  1989-03-15       Impact factor: 3.688

3.  Genetic map of Saccharomyces cerevisiae, edition 10.

Authors:  R K Mortimer; D Schild; C R Contopoulou; J A Kans
Journal:  Yeast       Date:  1989 Sep-Oct       Impact factor: 3.239

4.  Expression of plasmid R388-encoded type II dihydrofolate reductase as a dominant selective marker in Saccharomyces cerevisiae.

Authors:  A Miyajima; I Miyajima; K Arai; N Arai
Journal:  Mol Cell Biol       Date:  1984-03       Impact factor: 4.272

5.  Secretion of foreign proteins from Saccharomyces cerevisiae directed by alpha-factor gene fusions.

Authors:  G A Bitter; K K Chen; A R Banks; P H Lai
Journal:  Proc Natl Acad Sci U S A       Date:  1984-09       Impact factor: 11.205

6.  Secretion of mature mouse interleukin-2 by Saccharomyces cerevisiae: use of a general secretion vector containing promoter and leader sequences of the mating pheromone alpha-factor.

Authors:  A Miyajima; M W Bond; K Otsu; K Arai; N Arai
Journal:  Gene       Date:  1985       Impact factor: 3.688

7.  Expression of heterologous genes in Saccharomyces cerevisiae from vectors utilizing the glyceraldehyde-3-phosphate dehydrogenase gene promoter.

Authors:  G A Bitter; K M Egan
Journal:  Gene       Date:  1984-12       Impact factor: 3.688

8.  Efficient secretion of Bacillus amyloliquefaciens alpha-amylase by [corrected] its own signal peptide from Saccharomyces cerevisiae host cells [corrected].

Authors:  L Ruohonen; P Hackman; P Lehtovaara; J K Knowles; S Keränen
Journal:  Gene       Date:  1987       Impact factor: 3.688

Review 9.  Cell interactions and regulation of cell type in the yeast Saccharomyces cerevisiae.

Authors:  G F Sprague; L C Blair; J Thorner
Journal:  Annu Rev Microbiol       Date:  1983       Impact factor: 15.500

10.  Expression of murine and human granulocyte-macrophage colony-stimulating factors in S. cerevisiae: mutagenesis of the potential glycosylation sites.

Authors:  A Miyajima; K Otsu; J Schreurs; M W Bond; J S Abrams; K Arai
Journal:  EMBO J       Date:  1986-06       Impact factor: 11.598
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  3 in total

1.  Expression of the Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene together with the Erwinia pectate lyase and polygalacturonase genes in Saccharomyces cerevisiae.

Authors:  P van Rensburg; W H van Zyl; I S Pretorius
Journal:  Curr Genet       Date:  1994-12       Impact factor: 3.886

2.  Growth rate influences MF alpha 1 promoter activity in MAT alpha Saccharomyces cerevisiae.

Authors:  N Kirk; P W Piper
Journal:  Appl Microbiol Biotechnol       Date:  1994-11       Impact factor: 4.813

3.  Construction of the complete rat fatty acid synthase cDNA and its expression in Saccharomyces cerevisiae.

Authors:  R Kupfer; F Beiche; M Schweizer
Journal:  Curr Genet       Date:  1996-02       Impact factor: 3.886
  3 in total

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