| Literature DB >> 8472367 |
J P Teare1, N A Punchard, J J Powell, P J Lumb, W D Mitchell, R P Thompson.
Abstract
An enzymatic recycling method has been applied to the measurement of total and oxidized glutathione with a centrifugal analyzer. When the reduced form of glutathione (GSH) was masked with 2-vinylpyridine to measure the oxidized glutathione (GSSG), the time to ensure full derivatization was three times longer than has been reported. The method is quick, simple, accurate, and precise (1.27% for GSH, 3.3% for GSSG intraassay CV; 2.15% for GSH, 5% for GSSG interassay CV), and the automation allows large numbers of samples to be conveniently assayed.Entities:
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Year: 1993 PMID： 8472367
Source DB: PubMed Journal: Clin Chem ISSN： 0009-9147 Impact factor: 8.327