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Literature DB >> 8468306

Effects of amino acid substitutions in the promoter -10 binding region of the sigma A factor on growth of Bacillus subtilis.

Abstract

On the bases of structural and functional information about the Bacillus subtilis sigma A protein and the techniques of site-directed mutagenesis, we constructed a B. subtilis sigA mutant (DB1005) which grows normally at 37 degrees C but is sensitive to higher temperatures. DNA sequencing analyses revealed that DB1005 has Ile-198-->Ala and Ile-202-->Ala amino acid substitutions in the alpha-helix of the 2.4 region of the sigma A protein. Western blotting (immunoblotting) revealed that this mutant sigma A protein is stable at both 37 and 49 degrees C. These results suggest that Ile-198 and Ile-202 separately or in combination are critical for the sigma A protein to be functional at the restrictive temperature.

Entities: Chemical Species

Mesh：

Substances：
Sigma Factor

Year: 1993 PMID： 8468306 PMCID： PMC204541 DOI： 10.1128/jb.175.8.2470-2474.1993

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

26 in total

1. Overproduction, purification, and characterization of Bacillus subtilis RNA polymerase sigma A factor.

Authors: B Y Chang; R H Doi
Journal: J Bacteriol Date: 1990-06 Impact factor: 3.490

2. Two amino acids in an RNA polymerase sigma factor involved in the recognition of adjacent base pairs in the -10 region of a cognate promoter.

Authors: D Daniels; P Zuber; R Losick
Journal: Proc Natl Acad Sci U S A Date: 1990-10 Impact factor: 11.205

Effects of amino acid substitutions in the promoter -10 binding region of the sigma A factor on growth of Bacillus subtilis.

1. Overproduction, purification, and characterization of Bacillus subtilis RNA polymerase sigma A factor.

2. Two amino acids in an RNA polymerase sigma factor involved in the recognition of adjacent base pairs in the -10 region of a cognate promoter.

3. Use of helical wheels to represent the structures of proteins and to identify segments with helical potential.

Review 4. Structure and function of bacterial sigma factors.

5. Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction.

6. Mutation changing the specificity of an RNA polymerase sigma factor.

7. Site-directed mutagenesis by overlap extension using the polymerase chain reaction.

8. The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis.

9. A mutant Escherichia coli sigma 70 subunit of RNA polymerase with altered promoter specificity.

10. Energetics of complementary side-chain packing in a protein hydrophobic core.

1. Genetic and physiological studies of Bacillus subtilis sigma A mutants defective in promoter melting.

2. Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum.

3. The response of a Bacillus subtilis temperature-sensitive sigA mutant to heat stress.