Literature DB >> 8466805

Flow cytometric detection of wild yeast in lager breweries.

L Jespersen1, S Lassen, M Jakobsen.   

Abstract

A flow cytometric method for detection of wild yeast infections in breweries is reported. It is based on selective enrichment in Malt extract Yeast extract Glucose Peptone broth (MYGP) at 37 degrees C and in MYGP with 200 ppm CuSO4 at 25 degrees C, staining with a fluorochrome precursor and flow cytometry. In experiments with several types of wild yeast isolated from breweries and two different strains of lager yeast it has been possible to detect one wild yeast per 10(6) culture yeast after 48-72 h of incubation and, in some cases, after 24 h.

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Year:  1993        PMID: 8466805     DOI: 10.1016/0168-1605(93)90202-r

Source DB:  PubMed          Journal:  Int J Food Microbiol        ISSN: 0168-1605            Impact factor:   5.277


  2 in total

Review 1.  Flow cytometry applications in the food industry.

Authors:  Jaume Comas-Riu; Núria Rius
Journal:  J Ind Microbiol Biotechnol       Date:  2009-06-26       Impact factor: 3.346

Review 2.  Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.

Authors:  H M Davey; D B Kell
Journal:  Microbiol Rev       Date:  1996-12
  2 in total

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