| Literature DB >> 8465961 |
J Schäfer1, D M Turnbull, H Reichmann.
Abstract
A rapid and sensitive fluorometric assay for the measurement of carnitine palmitoyltransferase (CPT) activity is described. In this assay the coenzyme A (CoA) liberated from palmitoyl-CoA by CPT reacts with N-(9-acridinyl)-maleimide to form a fluorescent product. By comparing the fluorescence intensities of external CoA standards, CPT activity can be determined. The coefficients of variation within-run and between-run of the method were 6.9 and 10%, respectively. The CPT activity of human muscle samples measured with this assay is comparable to those obtained with a commonly used radiochemical assay and CPT deficiency in skeletal muscle from a patient was detected by this new fluorometric assay.Entities:
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Year: 1993 PMID： 8465961 DOI： 10.1006/abio.1993.1081
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365