Literature DB >> 8465387

The development and validation of a simple antigen detection ELISA for Plasmodium falciparum malaria.

D W Taylor1, A Voller.   

Abstract

A double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) is described for the detection of Plasmodium falciparum antigen. The test is based on an immunoglobulin (Ig) M capture monoclonal antibody on the solid phase and an IgG monoclonal antibody conjugated to peroxidase. The simple test takes about 2.5 h to complete and, because it uses whole blood with no prior treatment, it is possible to process batches of 50-100 samples simultaneously. The test is specific to P.falciparum and has a sensitivity close to that usually achieved with Giemsa-stained blood films. The reagents employed are stable at refrigerator temperatures for over 6 months, and as the test is compatible with human immunodeficiency virus and hepatitis B surface antigen ELISAs it could be suitable for blood transfusion screening.

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Year:  1993        PMID： 8465387     DOI： 10.1016/0035-9203(93)90409-j

Source DB:  PubMed          Journal:  Trans R Soc Trop Med Hyg        ISSN： 0035-9203            Impact factor:   2.184


  8 in total

1.  Recombinant antibodies specific for the Plasmodium falciparum histidine-rich protein 2.

Authors:  Elisabeth Ravaoarisoa; Halima Zamanka; Thierry Fusai; Jacques Bellalou; Hugues Bedouelle; Odile Mercereau-Puijalon; Thierry Fandeur
Journal:  MAbs       Date:  2010-07-01       Impact factor: 5.857

2.  Detection of the Plasmodium falciparum antigen histidine-rich protein 2 in blood of pregnant women: implications for diagnosing placental malaria.

Authors:  R F Leke; R R Djokam; R Mbu; R J Leke; J Fogako; R Megnekou; S Metenou; G Sama; Y Zhou; T Cadigan; M Parra; D W Taylor
Journal:  J Clin Microbiol       Date:  1999-09       Impact factor: 5.948

3.  Determination of immunoreactivity ofPlasmodium falciparum antigens, serum dilutions and biomaterials.

Authors:  M M Mya; R K Saxena; A Roy; D N Rao
Journal:  Indian J Clin Biochem       Date:  2004-01

4.  Quantitative detection of PfHRP2 in saliva of malaria patients in the Philippines.

Authors:  Andrew O Fung; Robert Damoiseaux; Sarah Grundeen; Jonnas L Panes; Daniel H Horton; Jack W Judy; Theodore B Moore
Journal:  Malar J       Date:  2012-05-25       Impact factor: 2.979

5.  Soluble Tumor Necrosis Factor-α Receptor 2 in Urine Is a Potential Biomarker for Noninvasive Diagnosis of Malaria During Pregnancy.

Authors:  Samuel Tassi Yunga; Audrey Davidson Thévenon; Rose Gana Fomban Leke; Diane Wallace Taylor
Journal:  Open Forum Infect Dis       Date:  2016-04-26       Impact factor: 3.835

6.  Individuals living in a malaria-endemic area of Cameroon do not have an acquired antibody response to Plasmodium falciparum histidine-rich protein 2.

Authors:  Diane Wallace Taylor; Naveen Bobbili; Vedbar S Khadka; Isabella A Quakyi; Rose G F Leke
Journal:  Malar J       Date:  2017-02-01       Impact factor: 2.979

7.  Enzyme-linked immunosorbent assay for detection of Plasmodium falciparum histidine-rich protein 2 in blood, plasma, and serum.

Authors:  Carolyne M Kifude; Halli G Rajasekariah; David J Sullivan; V Ann Stewart; Evelina Angov; Samuel K Martin; Carter L Diggs; John N Waitumbi
Journal:  Clin Vaccine Immunol       Date:  2008-03-26

8.  Detection of histidine rich protein & lactate dehydrogenase of Plasmodium falciparum in malaria patients by sandwich ELISA using in-house reagents.

Authors:  Priyanka Verma; Sukla Biswas; Teena Mohan; Shakir Ali; D N Rao
Journal:  Indian J Med Res       Date:  2013-12       Impact factor: 2.375
  8 in total

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