Literature DB >> 8464053

Imaging frozen, hydrated acrosomal bundle from Limulus sperm at 7 A resolution with a 400 kV electron cryomicroscope.

M F Schmid1, J Jakana, P Matsudaira, W Chiu.   

Abstract

Actin and scruin are present in a highly ordered array in the acrosomal bundle from Limulus sperm. Frozen, hydrated acrosomal bundle can be imaged in a 400 kV electron cryomicroscope using the spot-scan technique. Quantitative analysis of the h0l projection images shows diffraction data beyond 7 A.

Mesh:

Year:  1993        PMID: 8464053     DOI: 10.1006/jmbi.1993.1155

Source DB:  PubMed          Journal:  J Mol Biol        ISSN: 0022-2836            Impact factor:   5.469


  7 in total

1.  Imaging protein structure in water at 2.7 nm resolution by transmission electron microscopy.

Authors:  Utkur M Mirsaidov; Haimei Zheng; Yosune Casana; Paul Matsudaira
Journal:  Biophys J       Date:  2012-02-21       Impact factor: 4.033

2.  Three-dimensional structure of the acrosomal filament of Limulus sperm by 400 kV electron cryomicroscopy.

Authors:  M F Schmid; J Jakana; P Matsudaira; W Chiu
Journal:  Biophys J       Date:  1995-04       Impact factor: 4.033

3.  ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein.

Authors:  M C Hernandez; P J Andres-Barquin; S Martinez; A Bulfone; J L Rubenstein; M A Israel
Journal:  J Neurosci       Date:  1997-05-01       Impact factor: 6.167

4.  Three-dimensional structure of a single filament in the Limulus acrosomal bundle: scruin binds to homologous helix-loop-beta motifs in actin.

Authors:  M F Schmid; J M Agris; J Jakana; P Matsudaira; W Chiu
Journal:  J Cell Biol       Date:  1994-02       Impact factor: 10.539

5.  Determination of the alpha-actinin-binding site on actin filaments by cryoelectron microscopy and image analysis.

Authors:  A McGough; M Way; D DeRosier
Journal:  J Cell Biol       Date:  1994-07       Impact factor: 10.539

6.  Sequence and domain organization of scruin, an actin-cross-linking protein in the acrosomal process of Limulus sperm.

Authors:  M Way; M Sanders; C Garcia; J Sakai; P Matsudaira
Journal:  J Cell Biol       Date:  1995-01       Impact factor: 10.539

7.  Structural mechanism for bidirectional actin cross-linking by T-plastin.

Authors:  Lin Mei; Matthew J Reynolds; Damien Garbett; Rui Gong; Tobias Meyer; Gregory M Alushin
Journal:  Proc Natl Acad Sci U S A       Date:  2022-09-06       Impact factor: 12.779

  7 in total

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