Literature DB >> 8460489

Characterization of the in vitro system for the synthesis of mRNA from human respiratory syncytial virus.

Y T Huang1, R R Romito, B P De, A K Banerjee.   

Abstract

An in vitro transcription system for human respiratory syncytial virus (RSV) is described. Purified viral nucleocapsid (RNP) isolated from virus-infected cells was shown to support transcription of all 10 genes encoded by the virus as determined by Northern blot hybridization. The mRNAs synthesized were polyadenylated and comigrated with the corresponding mRNAs synthesized in virus-infected cells when analyzed in agarose-urea gel electrophoresis. The in vitro-synthesized mRNAs are functional as determined by their capacity to synthesize protein in vitro. The transcriptional reaction was significantly stimulated by the uninfected host cell lysate, indicating a requirement of host factor(s) in mRNA synthesis. Preliminary results suggest that cellular actin is involved in this process.

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Year:  1993        PMID： 8460489     DOI： 10.1006/viro.1993.1195

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  22 in total

1.  RhoA interacts with the fusion glycoprotein of respiratory syncytial virus and facilitates virus-induced syncytium formation.

Authors:  M K Pastey; J E Crowe; B S Graham
Journal:  J Virol       Date:  1999-09       Impact factor: 5.103

Review 2.  In vitro capping and transcription of rhabdoviruses.

Authors:  Tomoaki Ogino
Journal:  Methods       Date:  2012-06-08       Impact factor: 3.608

3.  Mutational analysis reveals a noncontractile but interactive role of actin and profilin in viral RNA-dependent RNA synthesis.

Authors:  Mary Harpen; Tiasha Barik; Alla Musiyenko; Sailen Barik
Journal:  J Virol       Date:  2009-08-26       Impact factor: 5.103

4.  Chimeric recombinant human metapneumoviruses with the nucleoprotein or phosphoprotein open reading frame replaced by that of avian metapneumovirus exhibit improved growth in vitro and attenuation in vivo.

Authors:  Quynh N Pham; Stéphane Biacchesi; Mario H Skiadopoulos; Brian R Murphy; Peter L Collins; Ursula J Buchholz
Journal:  J Virol       Date:  2005-12       Impact factor: 5.103

5.  Positive and negative host factors for Sendai virus transcription and their organ distribution in rat.

Authors:  T Takagi; M Iwama; K Seta; T Kanda; T Tsukamoto; S Tominaga; K Mizumoto
Journal:  Arch Virol       Date:  1996       Impact factor: 2.574

6.  Inhibitors of respiratory syncytial virus replication target cotranscriptional mRNA guanylylation by viral RNA-dependent RNA polymerase.

Authors:  Michel Liuzzi; Stephen W Mason; Mireille Cartier; Carol Lawetz; Robert S McCollum; Nathalie Dansereau; Gordon Bolger; Nicole Lapeyre; Yvon Gaudette; Lisette Lagacé; Marie-Josée Massariol; Florence Dô; Paul Whitehead; Lyne Lamarre; Erika Scouten; Josée Bordeleau; Serge Landry; Jean Rancourt; Gulrez Fazal; Bruno Simoneau
Journal:  J Virol       Date:  2005-10       Impact factor: 5.103

7.  Role of matrix and fusion proteins in budding of Sendai virus.

Authors:  T Takimoto; K G Murti; T Bousse; R A Scroggs; A Portner
Journal:  J Virol       Date:  2001-12       Impact factor: 5.103

Review 8.  Molecular mechanisms driving respiratory syncytial virus assembly.

Authors:  Fyza Y Shaikh; James E Crowe
Journal:  Future Microbiol       Date:  2013-01       Impact factor: 3.165

9.  Expression of L protein of vesicular stomatitis virus Indiana serotype from recombinant baculovirus in insect cells: requirement of a host factor(s) for its biological activity in vitro.

Authors:  M Mathur; T Das; A K Banerjee
Journal:  J Virol       Date:  1996-04       Impact factor: 5.103

10.  Polyadenylation-dependent screening assay for respiratory syncytial virus RNA transcriptase activity and identification of an inhibitor.

Authors:  Stephen W Mason; Carol Lawetz; Yvon Gaudette; Florence Dô; Erika Scouten; Lisette Lagacé; Bruno Simoneau; Michel Liuzzi
Journal:  Nucleic Acids Res       Date:  2004-09-08       Impact factor: 16.971
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