OBJECTIVE: To characterize the binding properties and variable-region sequences of LG4-1, a monoclonal antibody from an autoimmune MRL/Mp-lpr/lpr mouse that reacts specifically with nucleosome core particles and represents a new antinuclear antibody specificity. METHODS: The reactivity of the antibody against various nuclear substrates was determined using an enzymatic immunoassay, and the variable-region genes were sequenced from messenger RNA, using the dideoxy chain termination method. RESULTS: LG4-1 was found to react with nucleosome core particles but not with individual histones and DNA, or with various histone-histone and histone-DNA complexes. It was demonstrated that this antibody is encoded by a combination of variable-region genes and gene segments that have undergone few somatic mutations. CONCLUSION: The nucleosome core particle expresses a unique conformational autoepitope(s) resulting from the ordered association of histones and DNA.
OBJECTIVE: To characterize the binding properties and variable-region sequences of LG4-1, a monoclonal antibody from an autoimmune MRL/Mp-lpr/lprmouse that reacts specifically with nucleosome core particles and represents a new antinuclear antibody specificity. METHODS: The reactivity of the antibody against various nuclear substrates was determined using an enzymatic immunoassay, and the variable-region genes were sequenced from messenger RNA, using the dideoxy chain termination method. RESULTS: LG4-1 was found to react with nucleosome core particles but not with individual histones and DNA, or with various histone-histone and histone-DNA complexes. It was demonstrated that this antibody is encoded by a combination of variable-region genes and gene segments that have undergone few somatic mutations. CONCLUSION: The nucleosome core particle expresses a unique conformational autoepitope(s) resulting from the ordered association of histones and DNA.
Authors: Indira Neeli; Mekel M Richardson; Salar N Khan; Danielle Nicolo; Marc Monestier; Marko Z Radic Journal: Mol Immunol Date: 2006-11-03 Impact factor: 4.407
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Authors: Katy A Lloyd; Gustaf Wigerblad; Peter Sahlström; Manasa G Garimella; Karine Chemin; Johanna Steen; Philip J Titcombe; Bianka Marklein; Diana Zhou; Ragnhild Stålesen; Elena Ossipova; Christina Lundqvist; Olov Ekwall; Johan Rönnelid; Daniel L Mueller; Mikael C I Karlsson; Mariana J Kaplan; Karl Skriner; Lars Klareskog; Fredrik Wermeling; Vivianne Malmström; Caroline Grönwall Journal: Front Immunol Date: 2019-01-04 Impact factor: 7.561