Limitations of methods of osmometry: measuring the osmolality of biological fluids.

Osmometry is an important tool in the investigation of biological phenomena, and commercially available instruments for freezing point and vapor pressure osmometry can determine the osmolality of solutions quickly and inexpensively. However, accurate measurements of osmolality using these techniques require that the solutions have specific characteristics, and that measurements do not exceed the limitations inherent to each method or instrument. The thermodynamic principles underlying osmometry constrain the range and accuracy of each measurement method, and these must be considered in establishing the usefulness of each technique. This paper addresses the principles and limitations of routine osmometry techniques. We begin by discussing definitions of osmolality and the thermodynamic concepts of solute-solvent systems that are central to understanding osmometry of biological (i.e., aqueous) solutions. We then explore the application of various methods of measuring osmolality, the nature of errors introduced by overextension or misapplication of osmometry techniques, and the interpretation of data in the literature acquired by various methods and protocols.