Literature DB >> 8454198

Development of a selective and sensitive polymerase chain reaction assay for the detection of Mycoplasma pirum.

O Grau1, R Kovacic, R Griffais, L Montagnier.   

Abstract

A new assay using the polymerase chain reaction to amplify a 173-nucleotide DNA fragment within the 16S ribosomal RNA gene of Mycoplasma pirum has been developed. The assay selectively amplified DNA from all strains of M. pirum tested with a high level of sensitivity, even in a context of human DNA. DNA from other mollicute species, including those closely related to M. pirum, from bacteria phylogenetically close to mollicutes (Clostridium innocuum, C. ramosum and Bacillus subtilis), from Escherichia coli and from human peripheral blood mononuclear cells, did not produce the amplified DNA product specific for M. pirum.

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Year:  1993        PMID： 8454198     DOI： 10.1111/j.1574-6968.1993.tb05984.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  11 in total

1.  Mycoplasma penetrans and other mycoplasmas in urine of human immunodeficiency virus-positive children.

Authors:  A I Hussain; W L Robson; R Kelley; T Reid; J D Gangemi
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948

2.  Mycoplasmal infections prevent apoptosis and induce malignant transformation of interleukin-3-dependent 32D hematopoietic cells.

Authors:  S H Feng; S Tsai; J Rodriguez; S C Lo
Journal:  Mol Cell Biol       Date:  1999-12       Impact factor: 4.272

3.  Failure to detect Mycoplasma fermentans, Mycoplasma penetrans, or Mycoplasma pirum in the urethra of patients with acute nongonococcal urethritis.

Authors:  T Deguchi; C B Gilroy; D Taylor-Robinson
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1996-02       Impact factor: 3.267

4.  Mycoplasmas and oncogenesis: persistent infection and multistage malignant transformation.

Authors:  S Tsai; D J Wear; J W Shih; S C Lo
Journal:  Proc Natl Acad Sci U S A       Date:  1995-10-24       Impact factor: 11.205

5.  Detection of several Mycoplasma species at various anatomical sites of homosexual men.

Authors:  D Taylor-Robinson; C B Gilroy; F E Keane
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2003-05-07       Impact factor: 3.267

6.  Molecular characterization of the P1-like adhesin gene from Mycoplasma pirum.

Authors:  T N Tham; S Ferris; E Bahraoui; S Canarelli; L Montagnier; A Blanchard
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

7.  Rapid, sensitive PCR-based detection of mycoplasmas in simulated samples of animal sera.

Authors:  O Dussurget; D Roulland-Dussoix
Journal:  Appl Environ Microbiol       Date:  1994-03       Impact factor: 4.792

Review 8.  Molecular biology and pathogenicity of mycoplasmas.

Authors:  S Razin; D Yogev; Y Naot
Journal:  Microbiol Mol Biol Rev       Date:  1998-12       Impact factor: 11.056

9.  Search for the presence of six Mycoplasma species in peripheral blood mononuclear cells of subjects seropositive and seronegative for human immunodeficiency virus.

Authors:  R Kovacic; V Launay; P Tuppin; A Lafeuillade; V Feuillie; L Montagnier; O Grau
Journal:  J Clin Microbiol       Date:  1996-07       Impact factor: 5.948

10.  Antibiotic susceptibilities of AIDS-associated mycoplasmas.

Authors:  S A Poulin; R E Perkins; R B Kundsin
Journal:  J Clin Microbiol       Date:  1994-04       Impact factor: 5.948
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