Literature DB >> 8453610

Dual Alu polymerase chain reaction primers and conditions for isolation of human chromosome painting probes from hybrid cells.

P Liu1, J Siciliano, D Seong, J Craig, Y Zhao, P J de Jong, M J Siciliano.   

Abstract

A method for rapid and efficient production of chromosome- and chromosome-region specific probes for fluorescent in situ hybridization (FISH) detectable by simple fluorescent microscopy is described. The procedure is based on simultaneous use of two inter-Alu-polymerase chain reaction (PCR) primers for extraction of highly heterogeneous human DNA from interspecific somatic cell hybrids containing the chromosome regions of interest. Probes so produced do not hybridize to centromeric sequences and simultaneously band the target chromosomes, making them useful for unambiguous identification of chromosomal elements and breakpoints associated with cancer.

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Year:  1993        PMID： 8453610     DOI： 10.1016/0165-4608(93)90213-6

Source DB:  PubMed          Journal:  Cancer Genet Cytogenet        ISSN： 0165-4608


  12 in total

1.  Enhancing radiation hybrid mapping through whole genome amplification.

Authors:  Genesio M Karere; Leslie A Lyons; Lutz Froenicke
Journal:  Hereditas       Date:  2010-04       Impact factor: 3.271

2.  Identification of a gene that reverses the immortal phenotype of a subset of cells and is a member of a novel family of transcription factor-like genes.

Authors:  M J Bertram; N G Bérubé; X Hang-Swanson; Q Ran; J K Leung; S Bryce; K Spurgers; R J Bick; A Baldini; Y Ning; L J Clark; E K Parkinson; J C Barrett; J R Smith; O M Pereira-Smith
Journal:  Mol Cell Biol       Date:  1999-02       Impact factor: 4.272

Review 3.  Molecular cytogenetic analysis of eight inversion duplications of human chromosome 13q that each contain a neocentromere.

Authors:  P E Warburton; M Dolled; R Mahmood; A Alonso; S Li; K Naritomi; T Tohma; T Nagai; T Hasegawa; H Ohashi; L C Govaerts; B H Eussen; J O Van Hemel ; C Lozzio; S Schwartz; J J Dowhanick-Morrissette; N B Spinner; H Rivera; J A Crolla; C Yu; D Warburton
Journal:  Am J Hum Genet       Date:  2000-04-24       Impact factor: 11.025

4.  Molecular characterization of patients with 18q23 deletions.

Authors:  G Strathdee; R Sutherland; J J Jonsson; R Sataloff; M Kohonen-Corish; D Grady; J Overhauser
Journal:  Am J Hum Genet       Date:  1997-04       Impact factor: 11.025

5.  Correction of chromosomal instability and sensitivity to diverse mutagens by a cloned cDNA of the XRCC3 DNA repair gene.

Authors:  R S Tebbs; Y Zhao; J D Tucker; J B Scheerer; M J Siciliano; M Hwang; N Liu; R J Legerski; L H Thompson
Journal:  Proc Natl Acad Sci U S A       Date:  1995-07-03       Impact factor: 11.205

6.  Construction of neocentromere-based human minichromosomes by telomere-associated chromosomal truncation.

Authors:  R Saffery; L H Wong; D V Irvine; M A Bateman; B Griffiths; S M Cutts; M R Cancilla; A C Cendron; A J Stafford; K H Choo
Journal:  Proc Natl Acad Sci U S A       Date:  2001-05-01       Impact factor: 11.205

7.  Interstitial deletions are not the main mechanism leading to 18q deletions.

Authors:  G Strathdee; W Harrison; H C Riethman; S A Goodart; J Overhauser
Journal:  Am J Hum Genet       Date:  1994-06       Impact factor: 11.025

8.  A tumor suppressor locus within 3p14-p12 mediates rapid cell death of renal cell carcinoma in vivo.

Authors:  Y Sanchez; A el-Naggar; S Pathak; A M Killary
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-12       Impact factor: 11.205

9.  Tumor suppression and apoptosis of human prostate carcinoma mediated by a genetic locus within human chromosome 10pter-q11.

Authors:  Y Sanchez; M Lovell; M C Marin; P E Wong; M E Wolf-Ledbetter; T J McDonnell; A M Killary
Journal:  Proc Natl Acad Sci U S A       Date:  1996-03-19       Impact factor: 11.205

10.  Mutations of SURF-1 in Leigh disease associated with cytochrome c oxidase deficiency.

Authors:  V Tiranti; K Hoertnagel; R Carrozzo; C Galimberti; M Munaro; M Granatiero; L Zelante; P Gasparini; R Marzella; M Rocchi; M P Bayona-Bafaluy; J A Enriquez; G Uziel; E Bertini; C Dionisi-Vici; B Franco; T Meitinger; M Zeviani
Journal:  Am J Hum Genet       Date:  1998-12       Impact factor: 11.025
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