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Literature DB >> 845063

Demonstration of acetylcholinesterase by semipermeable membrane technique: estimation of soluble and fixation-labile portions in different regions of the central nervous system.

Abstract

A histochemical method for the demonstration of acetylcholinesterase using semipermeable membranes is described. This technique prevents any loss of enzyme activity caused by dissolution and/or fixation. The soluble and fixation-labile portions of acetylcholinesterase were estimated in several regions of the central nervous system of the rat and differences were found. The method improves the accuracy of the histochemical demonstration of the acetylcholinesterase on the light microscopical level.

Entities: Gene Species

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Year: 1977 PMID： 845063 DOI： 10.1007/BF00567228

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

32 in total

1. The subunit molecular weight of acetylcholinesterase.

Authors: David B. Millar; Melba A. Grafius
Journal: FEBS Lett Date: 1970-12-23 Impact factor: 4.124

2. Soluble and particle-bound acetylcholinesterase and its isoenzymes in peripheral nerves.

Authors: J Skangiel-Kramska; S Niemierko
Journal: J Neurochem Date: 1975-06 Impact factor: 5.372

3. Some aspects of fixation with glutaraldehyde. A biochemical and histochemical comparison of the effects of formaldehyde and glutaraldehyde fixation on various enzymes and glycogen, with a note on penetration of glutaraldehyde into liver.

Authors: D Hopwood
Journal: J Anat Date: 1967-01 Impact factor: 2.610

4. Changes in acetylcholinesterase and distribution of degenerating fibres in the hippocampal region after septal lesions in the rat.

Authors: S I Mellgren; B Srebro
Journal: Brain Res Date: 1973-03-30 Impact factor: 3.252

3. Histochemical demonstration of soluble and fixation-labile acetylcholinesterase activity in the optic tectum of rudd and frog with a semipermeable membrane technique.

Authors: J Andrä; Z Lojda; J Weiss; H Luppa
Journal: Histochem J Date: 1985-05

8. The multiple forms of brain acetycholinesterase. III. Implications for the histochemical demonstration of acetylcholinesterase.

Authors: G Hüther; H Luppa
Journal: Histochemistry Date: 1979-09

8 in total

Demonstration of acetylcholinesterase by semipermeable membrane technique: estimation of soluble and fixation-labile portions in different regions of the central nervous system.

1. The subunit molecular weight of acetylcholinesterase.

2. Soluble and particle-bound acetylcholinesterase and its isoenzymes in peripheral nerves.

3. Some aspects of fixation with glutaraldehyde. A biochemical and histochemical comparison of the effects of formaldehyde and glutaraldehyde fixation on various enzymes and glycogen, with a note on penetration of glutaraldehyde into liver.

4. Changes in acetylcholinesterase and distribution of degenerating fibres in the hippocampal region after septal lesions in the rat.

5. The release and molecular state of mammalian brain acetylcholinesterase.

6. Acetylcholinesterase in the feline cerebellum.

7. The localization of acetylcholinesterase in axonal membranes of frog nerve fibers.

8. Subcellular distribution of acetylcholinesterase activity in human brain caudate nucleus.

9. Confirmation from choline acetylase analyses of a massive cholinergic innervation to the rat hippocampus.

10. The cytological localization of intracellular neuronal acetylcholinesterase.

1. The multiple forms of brain acetylcholinesterase. I. Micro-electrophoresis and topochemical analysis of the pattern.

2. Histochemical demonstration of creatine kinase activity using polyvinyl alcohol and auxiliary enzymes.

3. Histochemical demonstration of soluble and fixation-labile acetylcholinesterase activity in the optic tectum of rudd and frog with a semipermeable membrane technique.

Review 4. The histochemistry of carboxylester hydrolases: problems and possibilities.

5. The multiple forms of brain acetylcholinesterase. II. A suggestion of their functional importance.

Review 6. Microelectrophoresis as a tool in enzyme histochemistry.

7. A histochemical method for the demonstration of acetylcholinesterase activity using semipermeable membranes.

8. The multiple forms of brain acetycholinesterase. III. Implications for the histochemical demonstration of acetylcholinesterase.