| Literature DB >> 8449830 |
T Yagyu1, T Monden, M Baba, Y Tamaki, T Takeda, T Kobayashi, T Shimano, Y Tsuji, H Matsushita, H Osawa.
Abstract
A human monoclonal antibody, YJ-37 (IgM) was generated through the fusion of human B lymphoblastoid cell line HO-323 with the regional lymph node lymphocytes from a colonic cancer patient who was treated with a local immunotherapy. This antibody was purified and conjugated with biotin, after which direct immunohistochemical staining was performed. The results revealed that YJ-37 selectively reacted with colonic cancer (7/19), gastric cancer (3/6), endometrial cancer (1/2) and colonic adenoma (7/13), but not with normal epithelia. Membrane immunofluorescence and FACS analysis also showed that YJ-37 bound to tumor cell surfaces. Furthermore, the chemical structure of the antigen defined by YJ-37 was analyzed by means of thin-layer chromatography immunostaining and ELISA. The results indicated that YJ-37 reacted with sialylated lacto-series carbohydrate chains, which have been reported to accumulate in cancer cells.Entities:
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Year: 1993 PMID： 8449830 PMCID： PMC5919026 DOI： 10.1111/j.1349-7006.1993.tb02787.x
Source DB: PubMed Journal: Jpn J Cancer Res ISSN： 0910-5050