A true microculture technique for human lymphocytes.

A true microculture technique utilizing only 0.01 ml of whole blood is presented. The blood is cultured in Eppendorf plastic microtubes in 1 ml of medium. This technique is easier, faster, and more economical than conventional methods. It is suitable for situations in which interphase cells or a small number (less than equal to 50) of metaphase cells are required.