Literature DB >> 8440391

Purification of recombinant hepatitis delta antigen expressed in E. coli cells.

R Calogero1, U Barbieri, M Borla, S Osborne, F Poisson, F Bonelli.   

Abstract

Recombinant DNA technology enables the massive production of recombinant hepatitis delta antigen (recHDAg) retaining immunological properties and transport functions. However, purification procedures of the recombinant delta antigen have, to date, not been described in the literature. We present a purification procedure allowing one to obtain highly purified recHDAg from bacterial cells expressing the hepatitis delta antigen.

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Year:  1993        PMID: 8440391     DOI: 10.1016/0014-5793(93)80538-6

Source DB:  PubMed          Journal:  FEBS Lett        ISSN: 0014-5793            Impact factor:   4.124


  1 in total

1.  Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris.

Authors:  Stephanie P Cartwright; Roslyn M Bill; Bui Tien Sy; Hieu Tran-Van; Hung Minh Nguyen
Journal:  BMC Res Notes       Date:  2017-07-27
  1 in total

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