Literature DB >> 8434953

Quantitative in vitro assay for human immunodeficiency virus deoxyribonucleic acid integration.

S Carteau1, J F Mouscadet, H Goulaouic, F Subra, C Auclair.   

Abstract

An obligatory step of retroviral growth is the integration of a DNA copy of the viral RNA into the genomic DNA of the host. Recombinant human immunodeficiency virus type I (HIV-1) integrase (IN) expressed in Escherichia coli efficiently catalyzes the overall in vitro integration reaction, namely, the processing of the long terminal repeat (LTR) ends and the strand transfer reaction. Using the 3' end of synthetic oligonucleotides which match the termini of HIV-1 LTRs as substrate and supercoiled pSP65 DNA as the target, we describe an assay that is suitable for the enzymatic analysis of the integration and for testing candidate inhibitors of HIV IN protein.

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Year:  1993        PMID： 8434953     DOI： 10.1006/abbi.1993.1105

Source DB:  PubMed          Journal:  Arch Biochem Biophys        ISSN： 0003-9861            Impact factor:   4.013


  6 in total

1.  Human immunodeficiency virus type 1 nucleocapsid protein specifically stimulates Mg2+-dependent DNA integration in vitro.

Authors:  S Carteau; S C Batson; L Poljak; J F Mouscadet; H de Rocquigny; J L Darlix; B P Roques; E Käs; C Auclair
Journal:  J Virol       Date:  1997-08       Impact factor: 5.103

2.  Differential divalent cation requirements uncouple the assembly and catalytic reactions of human immunodeficiency virus type 1 integrase.

Authors:  D J Hazuda; P J Felock; J C Hastings; B Pramanik; A L Wolfe
Journal:  J Virol       Date:  1997-09       Impact factor: 5.103

3.  Structural properties of HIV integrase. Lens epithelium-derived growth factor oligomers.

Authors:  Kushol Gupta; Tracy Diamond; Young Hwang; Frederic Bushman; Gregory D Van Duyne
Journal:  J Biol Chem       Date:  2010-04-20       Impact factor: 5.157

4.  Triterpene derivatives that block entry of human immunodeficiency virus type 1 into cells.

Authors:  J F Mayaux; A Bousseau; R Pauwels; T Huet; Y Hénin; N Dereu; M Evers; F Soler; C Poujade; E De Clercq
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-26       Impact factor: 11.205

5.  In vitro murine leukemia retroviral integration and structure fluctuation of target DNA.

Authors:  Tatsuaki Tsuruyama; Weizhi Liu; Kenichi Yoshikawa
Journal:  PLoS One       Date:  2012-02-14       Impact factor: 3.240

6.  In vitro HIV-1 LTR integration into T-cell activation gene CD27 segment and the decoy effect of modified-sequence DNA.

Authors:  Rei Ohmori; Tatsuaki Tsuruyama
Journal:  PLoS One       Date:  2012-11-28       Impact factor: 3.240
  6 in total

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