Literature DB >> 8432831

Effect of gamma irradiation on reactivity of rinderpest virus antigen with bovine immune serum in enzyme-linked immunosorbent assays and virus neutralization and indirect fluorescent-antibody tests.

J T Saliki1, M L Berninger, A Torres, J A House, C A Mebus, E J Dubovi.   

Abstract

Gamma irradiation effectively inactivated gradient-purified rinderpest virus. Irradiated antigen and sera remained functional in enzyme-linked immunosorbent assays, virus neutralization tests, and indirect fluorescent-antibody tests. Irradiation, however, led to a dose-dependent decrease in reactivity, particularly significant (P < 0.05) when both reagents were irradiated. To avoid false-positive reactions, only one reagent (serum or antigen) may be irradiated.

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Year:  1993        PMID： 8432831      PMCID： PMC262781          DOI： 10.1128/jcm.31.2.428-432.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Gamma radiation inactivation of coxsackievirus B-2.

Authors:  R Sullivan; P V Scarpino; A C Fassolitis; E P Larkin; J T Peeler
Journal:  Appl Microbiol       Date:  1973-07

2.  Gamma irradiation alters bluetongue virus protein antigen.

Authors:  A Afshar; P Moreau; G C Dulac; F C Thomas
Journal:  Acta Virol       Date:  1991-05       Impact factor: 1.162

3.  Gamma ray inactivation of some animal viruses.

Authors:  F C Thomas; A G Davies; G C Dulac; N G Willis; G Papp-Vid; A Girard
Journal:  Can J Comp Med       Date:  1981-10

4.  An enzyme-linked immunosorbent assay for the detection of IgG, IgA and IgM antibodies to rinderpest virus in experimentally infected cattle.

Authors:  J Anderson; L W Rowe; W P Taylor; J R Crowther
Journal:  Res Vet Sci       Date:  1982-03       Impact factor: 2.534

5.  Inactivation of African swine fever virus in tissues by gamma radiation.

Authors:  J W McVicar; C A Mebus; A Brynjolfsson; J S Walker
Journal:  Am J Vet Res       Date:  1982-02       Impact factor: 1.156

6.  Physico-chemical characterisation of rinderpest virus.

Authors:  B Underwood; F Brown
Journal:  Med Microbiol Immunol       Date:  1974       Impact factor: 3.402

7.  Inactivation of Lassa, Marburg, and Ebola viruses by gamma irradiation.

Authors:  L H Elliott; J B McCormick; K M Johnson
Journal:  J Clin Microbiol       Date:  1982-10       Impact factor: 5.948

8.  Inactivation of thirty viruses by gamma radiation.

Authors:  R Sullivan; A C Fassolitis; E P Larkin; R B Read; J T Peeler
Journal:  Appl Microbiol       Date:  1971-07

9.  Five measles virus antigens demonstrated by use of mouse hybridoma antibodies in productively infected tissue culture cells.

Authors:  E Norrby; S N Chen; T Togashi; H Shesberadaran; K P Johnson
Journal:  Arch Virol       Date:  1982       Impact factor: 2.574

10.  Inactivation by gamma irradiation of animal viruses in simulated laboratory effluent.

Authors:  F C Thomas; T Ouwerkerk; P McKercher
Journal:  Appl Environ Microbiol       Date:  1982-05       Impact factor: 4.792
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  2 in total

1.  Effect of two virus inactivation methods: electron beam irradiation and binary ethylenimine treatment on determination of reproductive hormones in equine plasma.

Authors:  N C Kyvsgaard; R Høier; I Brück; P Nansen
Journal:  Acta Vet Scand       Date:  1997       Impact factor: 1.695

2.  Activity of antibodies against Salmonella dublin, Toxoplasma gondii, or Actinobacillus pleuropneumoniae in sera after treatment with electron beam irradiation or binary ethylenimine.

Authors:  N C Kyvsgaard; P Lind; T Preuss; S Kamstrup; J C Lei; H O Bøgh; P Nansen
Journal:  Clin Diagn Lab Immunol       Date:  1996-11
  2 in total

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