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Literature DB >> 843164

Selective spore survival during replica-plating of fission yeast.

Abstract

Vegetative cells of Schizosaccharomyces pombe, upon exposure to acetone vapours, are inactivated at a faster rate than ascospores of this yeast. This observation has been used to develop a simple and fast method by which colonies can be replica-plated and only spores survive in the replicas. The colony patterns are exposed to acetone while still on the velvet used for replica-plating.

Entities: Chemical Species

Mesh：

Substances：
Acetone

Year: 1977 PMID： 843164 DOI： 10.1007/BF00446662

Source DB: PubMed Journal: Arch Microbiol ISSN： 0302-8933 Impact factor: 2.552

2 in total

1. [Physiologic and genetic studies of adenine-dependant mutants of Schizosaccharomyces pombe; a contribution to the problem of pseudoallele].

Authors: U LEUPOLD
Journal: Schweiz Z Pathol Bakteriol Date: 1957

2. Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether.

Authors: I W Dawes; I D Hardie
Journal: Mol Gen Genet Date: 1974

2 in total

12 in total

1. A double-strand break repair component is essential for S phase completion in fission yeast cell cycling.

Authors: K Suto; A Nagata; H Murakami; H Okayama
Journal: Mol Biol Cell Date: 1999-10 Impact factor: 4.138

2. Characterization of meiosis-deficient mutants by electron microscopy and mapping of four essential genes in the fission yeast Schizosaccharomyces pombe.

Authors: C Shimoda; A Hirata; M Kishida; T Hashida; K Tanaka
Journal: Mol Gen Genet Date: 1985

3. Orientation of "plus" genes at the mating-typing locus in homothallic fission yeast.

Authors: R Egal
Journal: Mol Gen Genet Date: 1978-05-31

4. ADAM family protein Mde10 is essential for development of spore envelopes in the fission yeast Schizosaccharomyces pombe.

Authors: Tomohiro Nakamura; Hiroko Abe; Aiko Hirata; Chikashi Shimoda
Journal: Eukaryot Cell Date: 2004-02

Selective spore survival during replica-plating of fission yeast.

1. [Physiologic and genetic studies of adenine-dependant mutants of Schizosaccharomyces pombe; a contribution to the problem of pseudoallele].

2. Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether.

1. A double-strand break repair component is essential for S phase completion in fission yeast cell cycling.

2. Characterization of meiosis-deficient mutants by electron microscopy and mapping of four essential genes in the fission yeast Schizosaccharomyces pombe.

3. Orientation of "plus" genes at the mating-typing locus in homothallic fission yeast.

4. ADAM family protein Mde10 is essential for development of spore envelopes in the fission yeast Schizosaccharomyces pombe.

5. Sequence requirement of the ade6-4095 meiotic recombination hotspot in Schizosaccharomyces pombe.

6. Novel nucleotide sequence motifs that produce hotspots of meiotic recombination in Schizosaccharomyces pombe.

7. The yeast spore wall enables spores to survive passage through the digestive tract of Drosophila.

8. The fission yeast spore is coated by a proteinaceous surface layer comprising mainly Isp3.

9. A genome-wide screen for sporulation-defective mutants in Schizosaccharomyces pombe.

10. Repeated evolution of self-compatibility for reproductive assurance.