Literature DB >> 8431287

Transformation of Helicobacter pylori by electroporation.

E D Segal1, L S Tompkins.   

Abstract

A highly efficient method of introducing DNA into an important human pathogen is reported here. Electroporation-mediated transformation of a laboratory-passaged isolate of Helicobacter pylori was successfully used to establish genetic mutants at a transformation frequency of > 10(5)/micrograms DNA. This method should be widely applicable to all isolates of H. pylori and may eliminate the variability reported when natural transformation was used on fresh clinical isolates.

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Year:  1993        PMID： 8431287

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  12 in total

1.  Phenotypic and genotypic variation in methylases involved in type II restriction-modification systems in Helicobacter pylori.

Authors:  Tohru Takata; Rahul Aras; Donald Tavakoli; Takafumi Ando; Asalia Z Olivares; Martin J Blaser
Journal:  Nucleic Acids Res       Date:  2002-06-01       Impact factor: 16.971

2.  Molecular characterization of a flagellar export locus of Helicobacter pylori.

Authors:  S Porwollik; B Noonan; P W O'Toole
Journal:  Infect Immun       Date:  1999-05       Impact factor: 3.441

3.  Evidence for a conjugation-like mechanism of DNA transfer in Helicobacter pylori.

Authors:  E J Kuipers; D A Israel; J G Kusters; M J Blaser
Journal:  J Bacteriol       Date:  1998-06       Impact factor: 3.490

4.  Analysis of F1F0-ATPase from Helicobacter pylori.

Authors:  C C McGowan; T L Cover; M J Blaser
Journal:  Infect Immun       Date:  1997-07       Impact factor: 3.441

5.  Functional and topological characterization of novel components of the comB DNA transformation competence system in Helicobacter pylori.

Authors:  Arno Karnholz; Claudia Hoefler; Stefan Odenbreit; Wolfgang Fischer; Dirk Hofreuter; Rainer Haas
Journal:  J Bacteriol       Date:  2006-02       Impact factor: 3.490

6.  Helicobacter pylori possesses two CheY response regulators and a histidine kinase sensor, CheA, which are essential for chemotaxis and colonization of the gastric mucosa.

Authors:  S Foynes; N Dorrell; S J Ward; R A Stabler; A A McColm; A N Rycroft; B W Wren
Journal:  Infect Immun       Date:  2000-04       Impact factor: 3.441

7.  HP0333, a member of the dprA family, is involved in natural transformation in Helicobacter pylori.

Authors:  T Ando; D A Israel; K Kusugami; M J Blaser
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

8.  Helicobacter pylori pore-forming cytolysin orthologue TlyA possesses in vitro hemolytic activity and has a role in colonization of the gastric mucosa.

Authors:  M C Martino; R A Stabler; Z W Zhang; M J Farthing; B W Wren; N Dorrell
Journal:  Infect Immun       Date:  2001-03       Impact factor: 3.441

9.  A urease-negative mutant of Helicobacter pylori constructed by allelic exchange mutagenesis lacks the ability to colonize the nude mouse stomach.

Authors:  M Tsuda; M Karita; M G Morshed; K Okita; T Nakazawa
Journal:  Infect Immun       Date:  1994-08       Impact factor: 3.441

10.  Isolation of the Helicobacter pylori recA gene and involvement of the recA region in resistance to low pH.

Authors:  S A Thompson; M J Blaser
Journal:  Infect Immun       Date:  1995-06       Impact factor: 3.441
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