G P Zaloga1, D Washburn, K W Black, R Prielipp. 1. Department of Anesthesia, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157-1009.
Abstract
OBJECTIVE: To determine whether free intracellular calcium is increased during human bacterial sepsis. DESIGN: Prospective controlled study of lymphocyte free intracellular calcium concentrations from patients with sepsis compared with critically ill nonseptic patients and healthy subjects. SETTING: A large multidisciplinary ICU of a university hospital. PATIENTS: Eleven patients with sepsis, six patients after cardiac surgery, six patients with head injury, and 22 healthy control subjects. INTERVENTIONS: Blood samples obtained for lymphocyte isolation and measurement of free intracellular calcium concentrations. MEASUREMENTS: Lymphocytes were isolated using Ficoll-paque centrifugation and free intracellular calcium concentrations were measured using the fluorescent dye fura-2. We also evaluated the effect of septic serum, endotoxin, tumor necrosis factor (TNF), and lysophosphatidylcholine on lymphocyte free intracellular calcium concentrations. MAIN RESULTS: Mean (+/- SEM) lymphocyte free intracellular calcium concentrations were significantly (p < .05) higher in the septic patients (176 +/- 12 nM) compared with cardiac surgical (112 +/- 9 nM), head-injured (110 +/- 11 nM), or healthy control patients (112 +/- 5 nM). Endotoxin (0.1 and 1.0 mg/mL) and TNF (10 and 100 ng/mL) did not alter lymphocyte free intracellular calcium values. Lysophosphatidylcholine (100 and 200 microM) significantly increased lymphocyte free intracellular calcium in a dose-dependent manner. Septic serum had no effect on resting lymphocyte free intracellular calcium concentrations but potentiated the free intracellular calcium response to lysophosphatidylcholine. CONCLUSIONS: Lymphocyte intracellular calcium homeostasis is altered during human sepsis. In addition, circulating factors present in septic serum are capable of altering cellular calcium handling.
OBJECTIVE: To determine whether free intracellular calcium is increased during human bacterial sepsis. DESIGN: Prospective controlled study of lymphocyte free intracellular calcium concentrations from patients with sepsis compared with critically ill nonseptic patients and healthy subjects. SETTING: A large multidisciplinary ICU of a university hospital. PATIENTS: Eleven patients with sepsis, six patients after cardiac surgery, six patients with head injury, and 22 healthy control subjects. INTERVENTIONS: Blood samples obtained for lymphocyte isolation and measurement of free intracellular calcium concentrations. MEASUREMENTS: Lymphocytes were isolated using Ficoll-paque centrifugation and free intracellular calcium concentrations were measured using the fluorescent dye fura-2. We also evaluated the effect of septic serum, endotoxin, tumor necrosis factor (TNF), and lysophosphatidylcholine on lymphocyte free intracellular calcium concentrations. MAIN RESULTS: Mean (+/- SEM) lymphocyte free intracellular calcium concentrations were significantly (p < .05) higher in the septic patients (176 +/- 12 nM) compared with cardiac surgical (112 +/- 9 nM), head-injured (110 +/- 11 nM), or healthy control patients (112 +/- 5 nM). Endotoxin (0.1 and 1.0 mg/mL) and TNF (10 and 100 ng/mL) did not alter lymphocyte free intracellular calcium values. Lysophosphatidylcholine (100 and 200 microM) significantly increased lymphocyte free intracellular calcium in a dose-dependent manner. Septic serum had no effect on resting lymphocyte free intracellular calcium concentrations but potentiated the free intracellular calcium response to lysophosphatidylcholine. CONCLUSIONS: Lymphocyte intracellular calcium homeostasis is altered during humansepsis. In addition, circulating factors present in septic serum are capable of altering cellular calcium handling.
Authors: Richard D Collage; Gina M Howell; Xianghong Zhang; Jennifer L Stripay; Janet S Lee; Derek C Angus; Matthew R Rosengart Journal: Crit Care Med Date: 2013-11 Impact factor: 7.598