BACKGROUND: The effect of enterohemorrhagic Escherichia coli O157:H7 infection on intestinal morphology and solute transport was examined. METHODS: New Zealand white rabbits, aged 10 days, were infected with E. coli strain EDL933 (O157:H7 containing the 60-megadalton plasmid-encoding adhesion factors VT1 and VT2) and compared with controls. Small and large intestinal histology and solute transport were studied 5 days after inoculation. Ion transport in the distal colon was also examined in animals infected with different strains encoding a combination of pathogenic factors. RESULTS: Infection with EDL933 induced diarrhea and mucosal disease in the colon, inhibited colonic Na+ absorption, and stimulated of Cl- secretion, but had no impact on the small intestine. Infection with strains A7785-C3A (O157:H7, plasmid-, VT1+, VT2+) and 85-170 (O157:H7, plasmid+, VT-) induced similar transport changes to EDL933. C600/1 (E. coli K-12, plasmid+, VT1+) decreased Na+ and Cl- absorption only. CONCLUSIONS: Abnormalities of colonic structure and ion transport could account for diarrhea production, but pathogenic factors other than the 60-megadalton plasmid-encoding adhesion factor and verotoxins appear to be involved in enterohemorrhagic E. coli infection.
BACKGROUND: The effect of enterohemorrhagic Escherichia coli O157:H7infection on intestinal morphology and solute transport was examined. METHODS: New Zealand white rabbits, aged 10 days, were infected with E. coli strain EDL933 (O157:H7 containing the 60-megadalton plasmid-encoding adhesion factors VT1 and VT2) and compared with controls. Small and large intestinal histology and solute transport were studied 5 days after inoculation. Ion transport in the distal colon was also examined in animals infected with different strains encoding a combination of pathogenic factors. RESULTS:Infection with EDL933 induced diarrhea and mucosal disease in the colon, inhibited colonic Na+ absorption, and stimulated of Cl- secretion, but had no impact on the small intestine. Infection with strains A7785-C3A (O157:H7, plasmid-, VT1+, VT2+) and 85-170 (O157:H7, plasmid+, VT-) induced similar transport changes to EDL933. C600/1 (E. coli K-12, plasmid+, VT1+) decreased Na+ and Cl- absorption only. CONCLUSIONS:Abnormalities of colonic structure and ion transport could account for diarrhea production, but pathogenic factors other than the 60-megadalton plasmid-encoding adhesion factor and verotoxins appear to be involved in enterohemorrhagic E. coli infection.
Authors: M Ogawa; K Shimizu; K Nomoto; M Takahashi; M Watanuki; R Tanaka; T Tanaka; T Hamabata; S Yamasaki; Y Takeda Journal: Infect Immun Date: 2001-02 Impact factor: 3.441