Literature DB >> 8424776

Purification of the pro-phenol oxidase enzyme from haemocytes of the cockroach Blaberus discoidalis.

H J Durrant1, N A Ratcliffe, C R Hipkin, A Aspan, K Söderhäll.   

Abstract

Pro-phenol oxidase was purified from the haemocytes of the cockroach Blaberus discoidalis by Blue Sepharose chromatography, hydrophobic-interaction chromatography on a Phenyl-Superose column and, finally, gel filtration on a Superose 6 column. Results suggest that the molecule exists as a polymer of identical 76 kDa monomeric units. The enzyme is a glycoprotein with pI of 5.2 and can be converted by trypsin into phenol oxidase.

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Year:  1993        PMID： 8424776      PMCID： PMC1132134          DOI： 10.1042/bj2890087

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  22 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  Purification of prophenoloxidase in the haemolymph of Calliphora vicina (R. & D.).

Authors:  S N Naqvi; P Karlson
Journal:  Arch Int Physiol Biochim       Date:  1979-10

3.  The majority of prophenoloxidase in the hemolymph of Manduca sexta is present in the plasma and not in the hemocytes.

Authors:  S J Saul; L Bin; M Sugumaran
Journal:  Dev Comp Immunol       Date:  1987       Impact factor: 3.636

4.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Microbial activation of prophenoloxidase from immune insect larvae.

Authors:  A E Pye
Journal:  Nature       Date:  1974-10-18       Impact factor: 49.962

7.  Hemocyte lysate enhancement of fungal spore encapsulation by crayfish hemocytes.

Authors:  K Söderhäll; A Vey; M Ramstedt
Journal:  Dev Comp Immunol       Date:  1984       Impact factor: 3.636

8.  Purification of hamster melanoma tyrosinases and structural studies of their asparagine-linked sugar chains.

Authors:  T Ohkura; K Yamashita; Y Mishima; A Kobata
Journal:  Arch Biochem Biophys       Date:  1984-11-15       Impact factor: 4.013

9.  Activation of pro-phenoloxidase by bacterial cell walls or beta-1,3-glucans in plasma of the silkworm, Bombyx mori.

Authors:  M Ashida; Y Ishizaki; H Iwahana
Journal:  Biochem Biophys Res Commun       Date:  1983-06-15       Impact factor: 3.575

10.  Genetics of a Drosophila phenoloxidase.

Authors:  T M Rizki; R M Rizki; R A Bellotti
Journal:  Mol Gen Genet       Date:  1985
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  4 in total

1.  Tyrosinases from crustaceans form hexamers.

Authors:  Elmar Jaenicke; Heinz Decker
Journal:  Biochem J       Date:  2003-04-15       Impact factor: 3.857

2.  Proenzyme of Manduca sexta phenol oxidase: purification, activation, substrate specificity of the active enzyme, and molecular cloning.

Authors:  M Hall; T Scott; M Sugumaran; K Söderhäll; J H Law
Journal:  Proc Natl Acad Sci U S A       Date:  1995-08-15       Impact factor: 11.205

3.  Density-Dependent Prophylaxis in Freshwater Snails Driven by Oxylipin Chemical Cues.

Authors:  Olwyn C Friesen; Chen-Hua Li; Ellen M E Sykes; Jake M Stout; Harold M Aukema; Ayush Kumar; Jillian T Detwiler
Journal:  Front Immunol       Date:  2022-01-31       Impact factor: 7.561

4.  Characterization of hemolymph phenoloxidase activity in two Biomphalaria snail species and impact of Schistosoma mansoni infection.

Authors:  Winka Le Clec'h; Timothy J C Anderson; Frédéric D Chevalier
Journal:  Parasit Vectors       Date:  2016-01-22       Impact factor: 3.876
  4 in total

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