Literature DB >> 8422994

Cloning and characterisation of an aminopeptidase P-encoding gene from Streptomyces lividans.

M J Butler1, A Bergeron, G Soostmeyer, T Zimny, L T Malek.   

Abstract

An aminopeptidase P (PepP)-encoding gene has been cloned from Streptomyces lividans 66 by screening for overexpression of activity using the chromogenic substrate Gly-Pro-beta-naphthylamide as a liquid overlayer on colonies growing on agar medium. The pepP gene was localised by deletion mapping, and the nucleotide sequence was determined. The deduced amino acid sequence was found to display significant similarity to Escherichia coli PepP. The partially purified S. lividans enzyme had a 50-kDa subunit and was present as a homodimer. Direct Edman degradation of the purified protein confirmed that pepP encoded the observed intracellular PepP.

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Year:  1993        PMID： 8422994     DOI： 10.1016/0378-1119(93)90549-i

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  11 in total

1.  Isolation and characterization of two genes encoding proteases associated with the mycelium of Streptomyces lividans 66.

Authors:  C Binnie; M J Butler; J S Aphale; R Bourgault; M A DiZonno; P Krygsman; L Liao; E Walczyk; L T Malek
Journal:  J Bacteriol       Date:  1995-11       Impact factor: 3.490

2.  Cloning and analysis of a gene from Streptomyces lividans 66 encoding a novel secreted protease exhibiting homology to subtilisin BPN'.

Authors:  M J Butler; J S Aphale; C Binnie; M A DiZonno; P Krygsman; G Soltes; E Walczyk; L T Malek
Journal:  Appl Microbiol Biotechnol       Date:  1996-03       Impact factor: 4.813

3.  Molecular cloning and expression in COS-1 cells of pig kidney aminopeptidase P.

Authors:  R J Hyde; N M Hooper; A J Turner
Journal:  Biochem J       Date:  1996-10-01       Impact factor: 3.857

4.  Intracellular aminopeptidases in Streptomyces lividans 66.

Authors:  M J Butler; J S Aphale; M A DiZonno; P Krygsman; E Walczyk; L T Malek
Journal:  J Ind Microbiol       Date:  1994-01

5.  Cloning and DNA sequence analysis of pepQ, a prolidase gene from Lactobacillus delbrueckii subsp. lactis DSM7290 and partial characterization of its product.

Authors:  K Stucky; J R Klein; A Schüller; H Matern; B Henrich; R Plapp
Journal:  Mol Gen Genet       Date:  1995-05-20

6.  Sequence and structure comparison suggest that methionine aminopeptidase, prolidase, aminopeptidase P, and creatinase share a common fold.

Authors:  J F Bazan; L H Weaver; S L Roderick; R Huber; B W Matthews
Journal:  Proc Natl Acad Sci U S A       Date:  1994-03-29       Impact factor: 11.205

7.  Eukaryotic methionyl aminopeptidases: two classes of cobalt-dependent enzymes.

Authors:  S M Arfin; R L Kendall; L Hall; L H Weaver; A E Stewart; B W Matthews; R A Bradshaw
Journal:  Proc Natl Acad Sci U S A       Date:  1995-08-15       Impact factor: 11.205

8.  Four novel PEPD alleles causing prolidase deficiency.

Authors:  P Ledoux; C Scriver; P Hechtman
Journal:  Am J Hum Genet       Date:  1994-06       Impact factor: 11.025

9.  Cloning and characterization of a gene encoding a secreted tripeptidyl aminopeptidase from Streptomyces lividans 66.

Authors:  M J Butler; C Binnie; M A DiZonno; P Krygsman; G A Soltes; G Soostmeyer; E Walczyk; L T Malek
Journal:  Appl Environ Microbiol       Date:  1995-08       Impact factor: 4.792

Review 10.  Interaction of antibacterial compounds with RND efflux pumps in Pseudomonas aeruginosa.

Authors:  Jürg Dreier; Paolo Ruggerone
Journal:  Front Microbiol       Date:  2015-07-08       Impact factor: 5.640
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