Literature DB >> 8422250

A new and rapid method for visualising DNA replication in spread DNA by immunofluorescence detection of incorporated 5-iododeoxyuridine.

N Tomilin1, Y u Rosanov, V Zenin, V Bozhkov, B Vig.   

Abstract

We have developed a new and rapid immunofluorescent method for visualisation of replicated regions on fixed DNA fibers. Using this method we have found in 5-fluorodeoxyuridine (FrdU)-blocked human cells distinct replication units covering about 60 kb of DNA and corresponding in size to single replicons or chromatin loops. Our results also suggest that nonadjacent replicons within a replicon cluster may be activated after FrdU arrest and that the method may be adapted for localization on fibers of specific DNA sequences.

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Year:  1993        PMID： 8422250     DOI： 10.1006/bbrc.1993.1039

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  5 in total

1.  Conformation of replicated segments of chromosome fibres in human S-phase nucleus.

Authors:  L Solovjeva; M Svetlova; G Stein; V Chagin; Y Rozanov; M Zannis-Hadjopoulos; G Price; N Tomilin
Journal:  Chromosome Res       Date:  1998-12       Impact factor: 5.239

2.  High mobility of flap endonuclease 1 and DNA polymerase eta associated with replication foci in mammalian S-phase nucleus.

Authors:  Lioudmila Solovjeva; Maria Svetlova; Lioudmila Sasina; Kyoji Tanaka; Masafumi Saijo; Igor Nazarov; Morton Bradbury; Nikolai Tomilin
Journal:  Mol Biol Cell       Date:  2005-03-09       Impact factor: 4.138

3.  Visualization of elementary DNA replication units in human nuclei corresponding in size to DNA loop domains.

Authors:  N Tomilin; L Solovjeva; R Krutilina; C Chamberland; R Hancock; B Vig
Journal:  Chromosome Res       Date:  1995-01       Impact factor: 5.239

4.  The structure of human S-phase chromosome fibres.

Authors:  M Svetlova; L Solovjeva; G Stein; C Chamberland; B Vig; N Tomilin
Journal:  Chromosome Res       Date:  1994-01       Impact factor: 5.239

5.  Replicon clusters are stable units of chromosome structure: evidence that nuclear organization contributes to the efficient activation and propagation of S phase in human cells.

Authors:  D A Jackson; A Pombo
Journal:  J Cell Biol       Date:  1998-03-23       Impact factor: 10.539
  5 in total

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