| Literature DB >> 8420802 |
K Hiraga1, K Suzuki, E Tsuchiya, T Miyakawa.
Abstract
A Saccharomyces cerevisiae cDNA homologue of the elongation factor EF-1 beta was found among the clones obtained by immunoscreening of a yeast cDNA expression library with an antibody against calmodulin affinity-purified proteins. The cDNA encoded a protein of 206 amino acids which was highly homologous (about 70% homology) with Artemia salina and human EF-1 beta. A protein with an apparent molecular mass of 33,000, significantly larger than that expected from the gene, was identified by Western blotting. Gene disruption experiments indicated that EF-1 beta is essential for growth.Entities:
Mesh：
Substances：
Year: 1993 PMID： 8420802 DOI： 10.1016/0014-5793(93)81208-h
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124