Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 MtrB from Bacillus subtilis binds specifically to trp leader RNA in a tryptophan-dependent manner.

Literature DB >> 8419914

MtrB from Bacillus subtilis binds specifically to trp leader RNA in a tryptophan-dependent manner.

Abstract

MtrB regulates transcription attenuation of the Bacillus subtilis trp operon. We have shown that MtrB, either from B. subtilis or overexpressed in Escherichia coli, binds specifically to RNA from the leader region of the trp operon by a gel mobility-shift assay. This binding is tryptophan dependent. MtrB binds to a transcript terminated at the trp attenuator (-2 to +138) or a read-through transcript (-2 to +318). MtrB does not bind antisense trp leader RNA or single-stranded trp leader DNA. These results support the model in which attenuation is controlled by tryptophan-activated MtrB influencing the secondary structure of the leader region transcript to form a terminator structure.

Entities: Chemical Species

Mesh：

Substances：

Year: 1993 PMID： 8419914 PMCID： PMC45613 DOI： 10.1073/pnas.90.1.128

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

23 in total

1. Novel form of transcription attenuation regulates expression the Bacillus subtilis tryptophan operon.

Authors: H Shimotsu; M I Kuroda; C Yanofsky; D J Henner
Journal: J Bacteriol Date: 1986-05 Impact factor: 3.490

2. RNA binding site of R17 coat protein.

Authors: P J Romaniuk; P Lowary; H N Wu; G Stormo; O C Uhlenbeck
Journal: Biochemistry Date: 1987-03-24 Impact factor: 3.162

3. Production of single-stranded plasmid DNA.

Authors: J Vieira; J Messing
Journal: Methods Enzymol Date: 1987 Impact factor: 1.600

4. Stabilization of the hexameric form of Escherichia coli protein rho under ATP hydrolysis conditions.

Authors: L R Finger; J P Richardson
Journal: J Mol Biol Date: 1982-03-25 Impact factor: 5.469

5. A convenient computer program for fitting enzymatic rate laws to steady-state data.

Authors: M C Kohn; L E Menten; D Garfinkel
Journal: Comput Biomed Res Date: 1979-10

6. Conformation of the TAR RNA-arginine complex by NMR spectroscopy.

Authors: J D Puglisi; R Tan; B J Calnan; A D Frankel; J R Williamson
Journal: Science Date: 1992-07-03 Impact factor: 47.728

7. Characterization of the Bacillus subtilis tryptophan promoter region.

Authors: H Shimotsu; D J Henner
Journal: Proc Natl Acad Sci U S A Date: 1984-10 Impact factor: 11.205

8. cis-acting sites in the transcript of the Bacillus subtilis trp operon regulate expression of the operon.

Authors: M I Kuroda; D Henner; C Yanofsky
Journal: J Bacteriol Date: 1988-07 Impact factor: 3.490

9. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

Authors: S Tabor; C C Richardson
Journal: Proc Natl Acad Sci U S A Date: 1985-02 Impact factor: 11.205

10. Reconstitution of Bacillus subtilis trp attenuation in vitro with TRAP, the trp RNA-binding attenuation protein.

Authors: P Babitzke; C Yanofsky
Journal: Proc Natl Acad Sci U S A Date: 1993-01-01 Impact factor: 11.205

44 in total

Review 1. Posttranscription initiation control of tryptophan metabolism in Bacillus subtilis by the trp RNA-binding attenuation protein (TRAP), anti-TRAP, and RNA structure.

Authors: P Babitzke; P Gollnick
Journal: J Bacteriol Date: 2001-10 Impact factor: 3.490

2. Population Distributions from Native Mass Spectrometry Titrations Reveal Nearest-Neighbor Cooperativity in the Ring-Shaped Oligomeric Protein TRAP.

Authors: Melody L Holmquist; Elihu C Ihms; Paul Gollnick; Vicki H Wysocki; Mark P Foster
Journal: Biochemistry Date: 2020-06-26 Impact factor: 3.162

3. A Bacillus subtilis operon containing genes of unknown function senses tRNATrp charging and regulates expression of the genes of tryptophan biosynthesis.

Authors: J P Sarsero; E Merino; C Yanofsky
Journal: Proc Natl Acad Sci U S A Date: 2000-03-14 Impact factor: 11.205

4. Translation control of trpG from transcripts originating from the folate operon promoter of Bacillus subtilis is influenced by translation-mediated displacement of bound TRAP, while translation control of transcripts originating from a newly identified trpG promoter is not.

Authors: Helen Yakhnin; Alexander V Yakhnin; Paul Babitzke
Journal: J Bacteriol Date: 2006-11-17 Impact factor: 3.490

5. NusA-stimulated RNA polymerase pausing and termination participates in the Bacillus subtilis trp operon attenuation mechanism invitro.

Authors: Alexander V Yakhnin; Paul Babitzke
Journal: Proc Natl Acad Sci U S A Date: 2002-08-02 Impact factor: 11.205

6. Effects of tryptophan starvation on levels of the trp RNA-binding attenuation protein (TRAP) and anti-TRAP regulatory protein and their influence on trp operon expression in Bacillus subtilis.

Authors: Wen-Jen Yang; Charles Yanofsky
Journal: J Bacteriol Date: 2005-03 Impact factor: 3.490

7. Translation of trpG in Bacillus subtilis is regulated by the trp RNA-binding attenuation protein (TRAP).

Authors: M Yang; A de Saizieu; A P van Loon; P Gollnick
Journal: J Bacteriol Date: 1995-08 Impact factor: 3.490

8. TRAP, the trp RNA-binding attenuation protein of Bacillus subtilis, is a toroid-shaped molecule that binds transcripts containing GAG or UAG repeats separated by two nucleotides.

Authors: P Babitzke; D G Bear; C Yanofsky
Journal: Proc Natl Acad Sci U S A Date: 1995-08-15 Impact factor: 11.205

9. trp RNA-binding attenuation protein (TRAP)-trp leader RNA interactions mediate translational as well as transcriptional regulation of the Bacillus subtilis trp operon.

Authors: E Merino; P Babitzke; C Yanofsky
Journal: J Bacteriol Date: 1995-11 Impact factor: 3.490

10. The pyrimidine biosynthesis operon of the thermophile Bacillus caldolyticus includes genes for uracil phosphoribosyltransferase and uracil permease.

Authors: S Y Ghim; J Neuhard
Journal: J Bacteriol Date: 1994-06 Impact factor: 3.490