Literature DB >> 8415558

Differentiation of Trichinella isolates by polymerase chain reaction.

C Soulé1, J P Guillou, J Dupouy-Camet, C Vallet, E Pozio.   

Abstract

Oligonucleotide primers were synthesized for the polymerase-chain-reaction amplification of target DNA from two sequences of Trichinella spiralis. Six strains belonging to T. spiralis, T. nativa, T. britovi, T. pseudospiralis, and T. nelsoni were tested. Amplification products were obtained with T. spiralis, T. britovi, and T. nelsoni DNA from a 53-kDa antigen cDNA sequence and with T. spiralis and T. nelsoni DNA from a 1.6-kb repetitive DNA sequence. Differences in the length of the amplification products obtained from the repetitive sequence would enable a differentiation between T. spiralis and T. nelsoni, suggesting that the 1.6 kb repetitive DNA sequence would not be specific for T. spiralis. No amplification was detected for T. nativa or T. pseudospiralis DNA from the two sequences and for T. britovi DNA from the 1.6-kb repetitive DNA sequence.

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Year:  1993        PMID： 8415558     DOI： 10.1007/bf00931583

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  22 in total

1.  Sequence analysis of a 1.6kb repetitive element from a porcine isolate of Trichinella spiralis.

Authors:  T deVos; G R Klassen; T A Dick
Journal:  Nucleic Acids Res       Date:  1988-04-11       Impact factor: 16.971

2.  A computer program for determining the size of DNA restriction fragments.

Authors:  R G Duggleby; H Kinns; J I Rood
Journal:  Anal Biochem       Date:  1981-01-01       Impact factor: 3.365

3.  Apple software for analysis of the size of restriction fragments.

Authors:  J I Rood; J M Gawthorne
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971

4.  Allozyme analysis of Trichinella isolates from various host species and geographical regions.

Authors:  G La Rosa; E Pozio; P Rossi; K D Murrell
Journal:  J Parasitol       Date:  1992-08       Impact factor: 1.276

5.  Taxonomic revision of the genus Trichinella.

Authors:  E Pozio; G La Rosa; K D Murrell; J R Lichtenfels
Journal:  J Parasitol       Date:  1992-08       Impact factor: 1.276

6.  Molecular cloning and expression of an immunodominant 53-kDa excretory-secretory antigen from Trichinella spiralis muscle larvae.

Authors:  D S Zarlenga; H R Gamble
Journal:  Mol Biochem Parasitol       Date:  1990 Sep-Oct       Impact factor: 1.759

7.  The use of enzyme polymorphisms to identify genetic differences in the genus Trichinella.

Authors:  L J Mydynski; T A Dick
Journal:  J Parasitol       Date:  1985-10       Impact factor: 1.276

8.  A repetitive DNA probe specific for a North American sylvatic genotype of Trichinella.

Authors:  D S Zarlenga; F Al-Yaman; D J Minchella; G La Rosa
Journal:  Mol Biochem Parasitol       Date:  1991-10       Impact factor: 1.759

9.  Enzyme polymorphism in Trichinella.

Authors:  H A Flockhart; S E Harrison; A R Dobinson; E R James
Journal:  Trans R Soc Trop Med Hyg       Date:  1982       Impact factor: 2.184

10.  The use of the polymerase chain reaction to identify porcine isolates of Trichinella.

Authors:  T A Dick; M C Lu; T deVos; K Ma
Journal:  J Parasitol       Date:  1992-02       Impact factor: 1.276
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  5 in total

1.  Detection and identification of eight Trichinella genotypes by reverse line blot hybridization.

Authors:  Y B Rombout; S Bosch; J W Van Der Giessen
Journal:  J Clin Microbiol       Date:  2001-02       Impact factor: 5.948

2.  PCR-RFLP patterns of four isolates of Trichinella for rDNA ITS1 region.

Authors:  H S Kwon; M S Chung; K H Joo
Journal:  Korean J Parasitol       Date:  2001-03       Impact factor: 1.341

3.  Identification of Trichinella isolates with random amplified polymorphic DNA markers.

Authors:  J Dupouy-Camet; F Robert; J P Guillou; C Vallet; C Perret; C Soulé
Journal:  Parasitol Res       Date:  1994       Impact factor: 2.289

Review 4.  A review of trichinellosis in people and wildlife in Canada.

Authors:  G D Appleyard; A A Gajadhar
Journal:  Can J Public Health       Date:  2000 Jul-Aug

Review 5.  Applications of DNA amplification techniques in veterinary diagnostics.

Authors:  M Pfeffer; M Wiedmann; C A Batt
Journal:  Vet Res Commun       Date:  1995       Impact factor: 2.459
  5 in total

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