Literature DB >> 841325

Aequorin luminescence: relation of light emission to calcium concentration--a calcium-independent component.

D G Allen, J R Blinks, F G Prendergast.   

Abstract

Light emission from the calcium-sensitive bioluminescent protein aequorin was measured at calcium ion concentrations of 10(-9) to 10(-2) molar. At very low Ca2+ concentations, light emission is independent of calcium ion concentration. The maximum slope of the log-log plot of light as a function of calcium ion concentration is about 2.5. The complete relation is well described by a two-state model involving three calcium-binding sites.

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Year:  1977        PMID： 841325     DOI： 10.1126/science.841325

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  103 in total

1.  The calcium rhythms of different cell types oscillate with different circadian phases.

Authors:  N T Wood; A Haley; M Viry-Moussaïd; C H Johnson; A H van der Luit; A J Trewavas
Journal:  Plant Physiol       Date:  2001-02       Impact factor: 8.340

2.  Mitochondrial Ca(2+)-induced Ca(2+) release mediated by the Ca(2+) uniporter.

Authors:  M Montero; M T Alonso; A Albillos; J García-Sancho; J Alvarez
Journal:  Mol Biol Cell       Date:  2001-01       Impact factor: 4.138

3.  Modulation of cytosolic free calcium concentration by alpha 1-adrenoceptors in rat atrial cells.

Authors:  U Jahnel; H Nawrath; R C Shieh; V K Sharma; D J Williford; S S Sheu
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1992-07       Impact factor: 3.000

4.  Isolation, purification and partial characterization of tropomyosin and troponin subunits from the lobster tail muscle.

Authors:  A Miegel; T Kobayashi; Y Maéda
Journal:  J Muscle Res Cell Motil       Date:  1992-12       Impact factor: 2.698

5.  Measurement of intracellular calcium during the development and relaxation of tonic tension in sheep Purkinje fibres.

Authors:  D A Eisner; M Valdeolmillos
Journal:  J Physiol       Date:  1986-06       Impact factor: 5.182

6.  Semi-synthetic aequorins with improved sensitivity to Ca2+ ions.

Authors:  O Shimomura; B Musicki; Y Kishi
Journal:  Biochem J       Date:  1989-08-01       Impact factor: 3.857

7.  Fluctuations in intracellular calcium concentration and their effect on tonic tension in canine cardiac Purkinje fibres.

Authors:  A A Kort; E G Lakatta; E Marban; M D Stern; W G Wier
Journal:  J Physiol       Date:  1985-10       Impact factor: 5.182

8.  Variation in myoplasmic Ca2+ concentration during contraction and relaxation studied by the indicator fluo-3 in frog muscle fibres.

Authors:  C Caputo; K A Edman; F Lou; Y B Sun
Journal:  J Physiol       Date:  1994-07-01       Impact factor: 5.182

9.  Changes in tetanic and resting [Ca2+]i during fatigue and recovery of single muscle fibres from Xenopus laevis.

Authors:  J A Lee; H Westerblad; D G Allen
Journal:  J Physiol       Date:  1991-02       Impact factor: 5.182

10.  Tension responses of chemically skinned fibre bundles of the guinea-pig taenia caeci under varied ionic environments.

Authors:  M Iino
Journal:  J Physiol       Date:  1981-11       Impact factor: 5.182
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