Literature DB >> 8409466

Selection of anticoagulants for lymphocyte immunophenotyping. Effect of specimen age on results.

J K Nicholson1, T A Green.   

Abstract

In a multi-center study, whole blood specimens from 31 HIV-positive and 43 HIV-negative donors were collected in three different anticoagulants and assayed for lymphocyte subsets fresh (within 6 h), and 1 and 2 days later. Each center prepared the specimens by their routine whole blood lysis procedure, labeling with a recommended panel of two-color monoclonal antibody combinations. 1 day (up to about 30 h) after blood collection, the results obtained from blood collected in EDTA (ethylenediamine tetra-acetate), ACD (acid citrate dextrose), and heparin were similar to fresh. Up to 48 h, only ACD and heparin, not EDTA, yielded results similar to fresh specimens. These results were similar for both HIV-positive and -negative specimens.

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Year:  1993        PMID： 8409466     DOI： 10.1016/0022-1759(93)90103-e

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  8 in total

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2.  Enumeration of lymphocyte subsets using flow cytometry: Effect of storage before and after staining in a developing country setting.

Authors:  Sanju Jalla; Sunil Sazawal; Salkat Deb; Robert E Black; Satya Narayan Das; Archana Sarkar; Maharaj K Bhan
Journal:  Indian J Clin Biochem       Date:  2004-07

3.  Modifications in flow cytometric estimation of t cell subsets and b cells in peripheral blood to reduce the cost of investigation.

Authors:  Sanju Jalla; Sunil Sazawal; Saikat Deb; Robert E Black; Maharaj K Bhan
Journal:  Indian J Clin Biochem       Date:  2002-01

4.  Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials.

Authors:  Marta Bull; Deborah Lee; Jason Stucky; Ya-Lin Chiu; Abbe Rubin; Helen Horton; M Juliana McElrath
Journal:  J Immunol Methods       Date:  2007-02-28       Impact factor: 2.303

5.  Ethyleneglycol-bis-(beta-aminoethylether)tetraacetate as a blood anticoagulant: preservation of antigen-presenting cell function and antigen-specific proliferative response of peripheral blood mononuclear cells from stored blood.

Authors:  P Kumar; V Satchidanandam
Journal:  Clin Diagn Lab Immunol       Date:  2000-07

6.  Effect of delayed cell processing and cryopreservation on immunophenotyping in multicenter population studies.

Authors:  Bharat Thyagarajan; Helene Barcelo; Eileen Crimmins; David Weir; Sharon Minnerath; Sithara Vivek; Jessica Faul
Journal:  J Immunol Methods       Date:  2018-09-14       Impact factor: 2.303

7.  Optimized multiparametric flow cytometric analysis of circulating endothelial cells and their subpopulations in peripheral blood of patients with solid tumors: a technical analysis.

Authors:  Fangbin Zhou; Yaying Zhou; Ming Yang; Jinli Wen; Jun Dong; Wenyong Tan
Journal:  Cancer Manag Res       Date:  2018-03-08       Impact factor: 3.989

8.  T-helper type 1 bias in healthy people is associated with cytomegalovirus serology and atherosclerosis: the Multi-Ethnic Study of Atherosclerosis.

Authors:  Russell P Tracy; Margaret F Doyle; Nels C Olson; Sally A Huber; Nancy S Jenny; Reem Sallam; Bruce M Psaty; Richard A Kronmal
Journal:  J Am Heart Assoc       Date:  2013-05-20       Impact factor: 5.501
  8 in total

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