Literature DB >> 8407370

A new approach to the immunocytochemistry of cAMP. Initial characterization of antibodies against acrolein-fixed cAMP.

J De Vente1, J Schipper, H W Steinbusch.   

Abstract

A method is described to couple cyclic adenosine 3',5' monophosphate (cAMP) to a carrier protein by means of acrolein. Antibodies against this conjugate were raised in mice. These antibodies proved to be highly specific for acrolein-fixed cAMP in a gelatin model system. Slices (300 microns in thickness) from rat cerebral cortex were incubated in vitro and the dopaminergic control of adenylate cyclase activity was drug-manipulated. This manipulation was visualized by application of the cAMP-antisera on cryostat sections of the acrolein fixed slices.

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Year:  1993        PMID： 8407370     DOI： 10.1007/bf00274098

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  22 in total

1.  Atrial natriuretic peptide increases cyclic guanosine monophosphate immunoreactivity in the carotid body.

Authors:  Z Z Wang; L J Stensaas; W J Wang; B Dinger; J de Vente; S J Fidone
Journal:  Neuroscience       Date:  1992-07       Impact factor: 3.590

Review 2.  Cyclic nucleotide immunocytochemistry.

Authors:  A L Steiner; S H Ong; H J Wedner
Journal:  Adv Cyclic Nucleotide Res       Date:  1976

Review 3.  A quantitative approach to cross-reaction problems in immunocytochemistry.

Authors:  F Berkenbosch; F J Tilders
Journal:  Neuroscience       Date:  1987-12       Impact factor: 3.590

4.  Cyclic nucleotides in the rat caudate-putamen complex: histochemical characterization and effects of deafferentation and kainic acid infusion.

Authors:  M A Ariano; L L Butcher; M M Appleman
Journal:  Neuroscience       Date:  1980       Impact factor: 3.590

Review 5.  Two dopamine receptors: biochemistry, physiology and pharmacology.

Authors:  J C Stoof; J W Kebabian
Journal:  Life Sci       Date:  1984-12-03       Impact factor: 5.037

6.  Formaldehyde fixation of cGMP in distinct cellular pools and their recognition by different cGMP-antisera. An immunocytochemical study into the problem of serum specificity.

Authors:  J de Vente; J Schipper; H W Steinbusch
Journal:  Histochemistry       Date:  1989

7.  Immunocytochemistry of cGMP in the Cerebellum of the Immature, Adult, and Aged Rat: the Involvement of Nitric Oxide. A Micropharmacological Study.

Authors:  J. De Vente; J. G. J. M. Bol; H. S. Berkelmans; J. Schipper; H. M. W. Steinbusch
Journal:  Eur J Neurosci       Date:  1990       Impact factor: 3.386

8.  Localization of cGMP after infusion of ANP or nitroprusside in the maturing rat.

Authors:  R L Chevalier; R J Fern; M Garmey; S S el-Dahr; R A Gomez; J De Vente
Journal:  Am J Physiol       Date:  1992-03

9.  Cyclic nucleotide distribution within rat striatonigral neurons.

Authors:  M A Ariano; S K Ufkes
Journal:  Neuroscience       Date:  1983-05       Impact factor: 3.590

10.  Ultrastructural localization of cyclic GMP and cyclic AMP in rat striatum.

Authors:  M A Ariano; A I Matus
Journal:  J Cell Biol       Date:  1981-10       Impact factor: 10.539
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  1 in total

1.  Modulation of Low-Voltage-Activated Inward Current Permeable to Sodium and Calcium by DARPP-32 Drives Spontaneous Firing of Insect Octopaminergic Neurosecretory Cells.

Authors:  Bruno Lapied; Antoine Defaix; Maria Stankiewicz; Eléonore Moreau; Valérie Raymond
Journal:  Front Syst Neurosci       Date:  2017-05-19
  1 in total

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