Literature DB >> 8404060

Sequence-independent amplification and labeling of yeast artificial chromosomes for fluorescence in situ hybridization.

S K Bohlander1, R Espinosa, A A Fernald, J D Rowley, M M Le Beau, M O Díaz.   

Abstract

We have developed a method that allows reliable construction of high quality FISH probes from yeast artificial chromosomes (YACs) based on the separation of YACs by pulse-field gel electrophoresis and a rapid sequence-independent amplification procedure (SIA). These probes can be used to localize YACs on metaphase chromosomes and also with high efficiency, in interphase nuclei.

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Year:  1994        PMID： 8404060     DOI： 10.1159/000133612

Source DB:  PubMed          Journal:  Cytogenet Cell Genet        ISSN： 0301-0171


  8 in total

1.  Subtracted, unique-sequence, in situ hybridization: experimental and diagnostic applications.

Authors:  J M Davison; T W Morgan; B L Hsi; S Xiao; J A Fletcher
Journal:  Am J Pathol       Date:  1998-11       Impact factor: 4.307

2.  Characterization and application of soybean YACs to molecular cytogenetics.

Authors:  T Zhu; I Shi; R P Funke; P M Gresshoff; P Keim
Journal:  Mol Gen Genet       Date:  1996-09-25

3.  Molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases to 1-1.5 Mb and preparation of a PAC-based physical map.

Authors:  N Zhao; A Stoffel; P W Wang; J D Eisenbart; R Espinosa; R A Larson; M M Le Beau
Journal:  Proc Natl Acad Sci U S A       Date:  1997-06-24       Impact factor: 11.205

4.  A method for screening arrayed cosmid libraries with mega insert yeast artificial chromosomes.

Authors:  M H Dreyling; O I Olopade; S K Bohlander
Journal:  Nucleic Acids Res       Date:  1995-03-25       Impact factor: 16.971

5.  The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.

Authors:  M H Dreyling; J A Martinez-Climent; M Zheng; J Mao; J D Rowley; S K Bohlander
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-14       Impact factor: 11.205

6.  Breakage in the SNRPN locus in a balanced 46,XY,t(15;19) Prader-Willi syndrome patient.

Authors:  Y Sun; R D Nicholls; M G Butler; S Saitoh; B E Hainline; C G Palmer
Journal:  Hum Mol Genet       Date:  1996-04       Impact factor: 6.150

7.  Construction of a 2.8-megabase yeast artificial chromosome contig and cloning of the human methylthioadenosine phosphorylase gene from the tumor suppressor region on 9p21.

Authors:  O I Olopade; H M Pomykala; F Hagos; L W Sveen; R Espinosa; M H Dreyling; S Gursky; W M Stadler; M M Le Beau; S K Bohlander
Journal:  Proc Natl Acad Sci U S A       Date:  1995-07-03       Impact factor: 11.205

8.  Microdissection of the Y chromosome and fluorescence in situ hybridization analysis of the sex chromosomes of lake trout, Salvelinus namaycush.

Authors:  K M Reed; S K Bohlander; R B Phillips
Journal:  Chromosome Res       Date:  1995-06       Impact factor: 5.239
  8 in total

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