Literature DB >> 8400138

Characterization of pea chloroplastic carbonic anhydrase. Expression in Escherichia coli and site-directed mutagenesis.

N J Provart1, N Majeau, J R Coleman.   

Abstract

A cDNA encoding the mature, chloroplast-localized carbonic anhydrase in pea has been expressed in E. coli. The enzyme is fully active and yields of up to 20% of the total soluble protein can be obtained from the bacteria. This expression system was used to monitor the effects of site-directed mutagenesis of seven residues found within conserved regions in the pea carbonic anhydrase amino acid sequence. The effects of these modifications are discussed with respect to the potential of various amino acids to act as sites for zinc coordination or intramolecular proton shuttles.

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Year:  1993        PMID： 8400138     DOI： 10.1007/bf00028967

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  19 in total

1.  Nucleotide sequence of a complementary DNA encoding tobacco chloroplastic carbonic anhydrase.

Authors:  N Majeau; J R Coleman
Journal:  Plant Physiol       Date:  1992-10       Impact factor: 8.340

2.  Isolation and characterization of a cDNA coding for pea chloroplastic carbonic anhydrase.

Authors:  N Majeau; J R Coleman
Journal:  Plant Physiol       Date:  1991-01       Impact factor: 8.340

3.  Electrometric and colorimetric determination of carbonic anhydrase.

Authors:  K M WILBUR; N G ANDERSON
Journal:  J Biol Chem       Date:  1948-10       Impact factor: 5.157

4.  Quantitation of protein.

Authors:  C M Stoscheck
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

5.  A gene homologous to chloroplast carbonic anhydrase (icfA) is essential to photosynthetic carbon dioxide fixation by Synechococcus PCC7942.

Authors:  H Fukuzawa; E Suzuki; Y Komukai; S Miyachi
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-15       Impact factor: 11.205

6.  Rapid and efficient site-specific mutagenesis without phenotypic selection.

Authors:  T A Kunkel; J D Roberts; R A Zakour
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

7.  The role of the metal ion in the refolding of denatured bovine Co(II)-carbonic anhydrase II.

Authors:  N Bergenhem; U Carlsson
Journal:  Biochim Biophys Acta       Date:  1989-10-19

8.  Spinach chloroplastic carbonic anhydrase: nucleotide sequence analysis of cDNA.

Authors:  J N Burnell; M J Gibbs; J G Mason
Journal:  Plant Physiol       Date:  1990-01       Impact factor: 8.340

9.  Role of histidine 64 in the catalytic mechanism of human carbonic anhydrase II studied with a site-specific mutant.

Authors:  C K Tu; D N Silverman; C Forsman; B H Jonsson; S Lindskog
Journal:  Biochemistry       Date:  1989-09-19       Impact factor: 3.162

10.  Refined structure of human carbonic anhydrase II at 2.0 A resolution.

Authors:  A E Eriksson; T A Jones; A Liljas
Journal:  Proteins       Date:  1988
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  14 in total

1.  Loss of the Chloroplast Transit Peptide from an Ancestral C3 Carbonic Anhydrase Is Associated with C4 Evolution in the Grass Genus Neurachne.

Authors:  Harmony Clayton; Montserrat Saladié; Vivien Rolland; Robert Sharwood; Terry Macfarlane; Martha Ludwig
Journal:  Plant Physiol       Date:  2017-02-02       Impact factor: 8.340

2.  Crystal structure of E. coli beta-carbonic anhydrase, an enzyme with an unusual pH-dependent activity.

Authors:  J D Cronk; J A Endrizzi; M R Cronk; J W O'neill; K Y Zhang
Journal:  Protein Sci       Date:  2001-05       Impact factor: 6.725

3.  The active site architecture of Pisum sativum beta-carbonic anhydrase is a mirror image of that of alpha-carbonic anhydrases.

Authors:  M S Kimber; E F Pai
Journal:  EMBO J       Date:  2000-04-03       Impact factor: 11.598

Review 4.  Advances in understanding the physiological role and locations of carbonic anhydrases in C3 plant cells.

Authors:  Natalia N Rudenko; Lyudmila K Ignatova; Elena M Nadeeva-Zhurikova; Tatiana P Fedorchuk; Boris N Ivanov; Maria M Borisova-Mubarakshina
Journal:  Protoplasma       Date:  2020-10-28       Impact factor: 3.356

5.  Molecular comparison of carbonic anhydrase from Flaveria species demonstrating different photosynthetic pathways.

Authors:  M Ludwig; J N Burnell
Journal:  Plant Mol Biol       Date:  1995-10       Impact factor: 4.076

6.  Nucleotide sequence of a cDNA encoding rice chloroplastic carbonic anhydrase.

Authors:  S Suzuki; J N Burnell
Journal:  Plant Physiol       Date:  1995-01       Impact factor: 8.340

7.  Modification of carbonic anhydrase activity by antisense and over-expression constructs in transgenic tobacco.

Authors:  N Majeau; M A Arnoldo; J R Coleman
Journal:  Plant Mol Biol       Date:  1994-06       Impact factor: 4.076

8.  Loss of the transit peptide and an increase in gene expression of an ancestral chloroplastic carbonic anhydrase were instrumental in the evolution of the cytosolic C4 carbonic anhydrase in Flaveria.

Authors:  Sandra K Tanz; Sasha G Tetu; Nicole G F Vella; Martha Ludwig
Journal:  Plant Physiol       Date:  2009-05-15       Impact factor: 8.340

9.  The Flaveria bidentis beta-carbonic anhydrase gene family encodes cytosolic and chloroplastic isoforms demonstrating distinct organ-specific expression patterns.

Authors:  Sasha G Tetu; Sandra K Tanz; Nicole Vella; James N Burnell; Martha Ludwig
Journal:  Plant Physiol       Date:  2007-05-11       Impact factor: 8.340

10.  Complementation of the yeast deletion mutant DeltaNCE103 by members of the beta class of carbonic anhydrases is dependent on carbonic anhydrase activity rather than on antioxidant activity.

Authors:  Daniel Clark; Roger S Rowlett; John R Coleman; Daniel F Klessig
Journal:  Biochem J       Date:  2004-05-01       Impact factor: 3.857
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