Literature DB >> 8400133

Single-seed PCR of LMW glutenin genes to distinguish between durum wheat cultivars with good and poor technological properties.

R D'Ovidio1.   

Abstract

Polymerase chain reaction (PCR) was used to amplify low-molecular-weight (LMW) glutenin sequences from genomic DNA extracted from a single germinating seed of several durum wheat genotypes. Electrophoretic analysis of PCR reactions showed the presence of amplified products characteristic of durum wheat cultivars with good and poor technological properties. This PCR-based approach is proposed as a very efficient and safe alternative to standard procedures for selecting durum wheat genotypes with good qualitative characteristics.

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Year:  1993        PMID： 8400133     DOI： 10.1007/bf00028988

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  5 in total

1.  Rapid and efficient detection of genetic polymorphism in wheat through amplification by polymerase chain reaction.

Authors:  R D'Ovidio; O A Tanzarella; E Porceddu
Journal:  Plant Mol Biol       Date:  1990-07       Impact factor: 4.076

2.  A simple and rapid method for the preparation of plant genomic DNA for PCR analysis.

Authors:  K Edwards; C Johnstone; C Thompson
Journal:  Nucleic Acids Res       Date:  1991-03-25       Impact factor: 16.971

3.  Molecular characterization of an active wheat LMW glutenin gene and its relation to other wheat and barley prolamin genes.

Authors:  V Colot; D Bartels; R Thompson; R Flavell
Journal:  Mol Gen Genet       Date:  1989-03

4.  Rapid identification of Triticeae genotypes from single seeds using the polymerase chain reaction.

Authors:  C Benito; A M Figueiras; C Zaragoza; F J Gallego; A de la Peña
Journal:  Plant Mol Biol       Date:  1993-01       Impact factor: 4.076

5.  Chromosomal location of genes for gliadin polypeptides in durum wheat Triticum turgidum L.

Authors:  L R Joppa; K Khan; N D Williams
Journal:  Theor Appl Genet       Date:  1983-03       Impact factor: 5.699
  5 in total
  5 in total

1.  PCR-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (Triticum aestivum L.).

Authors:  Xiaofei Zhang; Dongcheng Liu; Wei Jiang; Xiaoli Guo; Wenlong Yang; Jiazhu Sun; Hongqing Ling; Aimin Zhang
Journal:  Theor Appl Genet       Date:  2011-08-10       Impact factor: 5.699

2.  Locus-specific primers for LMW glutenin genes on each of the group 1 chromosomes of hexaploid wheat.

Authors:  S Van Campenhout; J Vander Stappen; L Sagi; G Volckaert
Journal:  Theor Appl Genet       Date:  1995-07       Impact factor: 5.699

3.  Characterization of a low-molecular-weight glutenin subunit gene from bread wheat and the corresponding protein that represents a major subunit of the glutenin polymer.

Authors:  S Masci; R D'Ovidio; D Lafiandra; D D Kasarda
Journal:  Plant Physiol       Date:  1998-12       Impact factor: 8.340

4.  Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum. L).

Authors:  W Zhang; M C Gianibelli; L R Rampling; K R Gale
Journal:  Theor Appl Genet       Date:  2004-01-16       Impact factor: 5.699

5.  Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.).

Authors:  Xiaofei Zhang; Dongcheng Liu; Wenlong Yang; Kunfan Liu; Jiazhu Sun; Xiaoli Guo; Yiwen Li; Daowen Wang; Hongqing Ling; Aimin Zhang
Journal:  Theor Appl Genet       Date:  2011-02-23       Impact factor: 5.699
  5 in total

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