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Literature DB >> 8400121

Nuclease sensitivity and functional analysis of a maize histone H3 gene promoter.

P Brignon¹, M Lepetit, C Gigot, N Chaubet.

Abstract

A 1 kb region of a maize H3 histone gene promoter has been analysed at a structural and functional level. Micrococcal nuclease digestion of isolated nuclei showed that the promoter region is organized into nucleosomes but a zone extending over approximately one nucleosome (20 to 230 bp upstream of the TATA box) displays remarkable accessibility to digestion. Three DNase I-hypersensitive sites were found within this zone at the vicinity of consensus sequences, some of which are already known to act as cis elements. This promoter region is able to direct faithful expression of the GUS reporter gene in meristematic tissues of transgenic tobacco plants.

Entities: Disease Species

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Year: 1993 PMID： 8400121 DOI： 10.1007/bf00028973

Source DB: PubMed Journal: Plant Mol Biol ISSN： 0167-4412 Impact factor: 4.076

17 in total

1. Highly conserved hexamer, octamer and nonamer motifs are positive cis-regulatory elements of the wheat histone H3 gene.

Authors: T Nakayama; A Sakamoto; P Yang; M Minami; Y Fujimoto; T Ito; M Iwabuchi
Journal: FEBS Lett Date: 1992-03-30 Impact factor: 4.124

2. A plant histone gene promoter can direct both replication-dependent and -independent gene expression in transgenic plants.

Authors: M Lepetit; M Ehling; N Chaubet; C Gigot
Journal: Mol Gen Genet Date: 1992-01

3. A 126 bp fragment of a plant histone gene promoter confers preferential expression in meristems of transgenic Arabidopsis.

Authors: R Atanassova; N Chaubet; C Gigot
Journal: Plant J Date: 1992-05 Impact factor: 6.417

5. Functional analysis of the promoter region of a maize (Zea mays L.) H3 histone gene in transgenic Arabidopsis thaliana.

Authors: R Atanassova; M Flénet; C Gigot; N Chaubet
Journal: Plant Mol Biol Date: 1998-05 Impact factor: 4.076

5 in total

Nuclease sensitivity and functional analysis of a maize histone H3 gene promoter.

1. Highly conserved hexamer, octamer and nonamer motifs are positive cis-regulatory elements of the wheat histone H3 gene.

2. A plant histone gene promoter can direct both replication-dependent and -independent gene expression in transgenic plants.

3. A 126 bp fragment of a plant histone gene promoter confers preferential expression in meristems of transgenic Arabidopsis.

4. An internal standard improves the reliability of transient expression studies in plant protoplasts.

5. Tissue-specific expression of a wheat high molecular weight glutenin gene in transgenic tobacco.

Review 6. Multilevel regulation of replication-dependent histone genes.

7. The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.

8. DNase I hypersensitive sites in the 5'-region of the maize Shrunken gene in nuclei from different organs.

9. Cell-specific expression of plant histone H2A genes.

10. DNase I hypersensitivity and expression of the Shrunken-1 gene of maize.

Review 1. Regulation of histone gene expression during the cell cycle.

2. Histone H3 transcript stability in alfalfa.

3. Multilevel regulation of histone gene expression during the cell cycle in tobacco cells.

4. Analysis of chromatin structure in the control regions of the chlamydomonas HSP70A and RBCS2 genes.

5. Functional analysis of the promoter region of a maize (Zea mays L.) H3 histone gene in transgenic Arabidopsis thaliana.