Literature DB >> 8396947

Epstein-Barr virus-derived vectors for transient and stable expression of recombinant proteins.

G Cachianes1, C Ho, R F Weber, S R Williams, D V Goeddel, D W Leung.   

Abstract

The transient expression level of foreign proteins in primate cells can be enhanced by incorporating the replication elements derived from Epstein-Barr virus (EBV). Specifically, we have constructed expression plasmids with the replication origin region (OriP) from EBV and an adenovirus-transformed cell line that expresses the EBV nuclear antigens-1 (EBNA-1). As EBV vectors can replicate as episomes in the nuclei, such vectors can have a stable transfection efficiency as high as 25% and provide a straightforward way of obtaining large amounts of recombinant proteins transiently or stably.

Entities:  

Mesh：

Substances：

Year:  1993        PMID： 8396947

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  18 in total

1.  Enhanced CHO cell-based transient gene expression with the epi-CHO expression system.

Authors:  Joe Codamo; Trent P Munro; Benjamin S Hughes; Michael Song; Peter P Gray
Journal:  Mol Biotechnol       Date:  2011-06       Impact factor: 2.695

2.  Development of a generic transient transfection process at 100 L scale.

Authors:  Ola Tuvesson; Christina Uhe; Aleksei Rozkov; Elke Lüllau
Journal:  Cytotechnology       Date:  2008-02-22       Impact factor: 2.058

3.  In vitro and in vivo analysis of the interaction between RNA helicase A and HIV-1 RNA.

Authors:  Li Xing; Meijuan Niu; Lawrence Kleiman
Journal:  J Virol       Date:  2012-09-26       Impact factor: 5.103

Review 4.  Large-scale transfection of mammalian cells for the fast production of recombinant protein.

Authors:  Phuong Lan Pham; Amine Kamen; Yves Durocher
Journal:  Mol Biotechnol       Date:  2006-10       Impact factor: 2.695

5.  High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells.

Authors:  Yves Durocher; Sylvie Perret; Amine Kamen
Journal:  Nucleic Acids Res       Date:  2002-01-15       Impact factor: 16.971

Review 6.  Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules.

Authors:  Isabelle Legastelois; Sophie Buffin; Isabelle Peubez; Charlotte Mignon; Régis Sodoyer; Bettina Werle
Journal:  Hum Vaccin Immunother       Date:  2016-12-01       Impact factor: 3.452

7.  Prospects for the use of artificial chromosomes and minichromosome-like episomes in gene therapy.

Authors:  Sara Pérez-Luz; Javier Díaz-Nido
Journal:  J Biomed Biotechnol       Date:  2010-08-24

8.  CT-2576, an inhibitor of phospholipid signaling, suppresses constitutive and induced expression of human immunodeficiency virus.

Authors:  D W Leung; P K Peterson; R Weeks; G Gekker; C C Chao; A H Kaplan; N Balantac; C Tompkins; G E Underiner; S Bursten
Journal:  Proc Natl Acad Sci U S A       Date:  1995-05-23       Impact factor: 11.205

9.  Transient transfection factors for high-level recombinant protein production in suspension cultured mammalian cells.

Authors:  Chaoting Liu; Brian Dalby; Weixing Chen; Jennifer M Kilzer; Henry C Chiou
Journal:  Mol Biotechnol       Date:  2008-06       Impact factor: 2.695

10.  An oriP expression vector containing the HIV-1 Tat/TAR transactivation axis produces high levels of protein expression in mammalian cells.

Authors:  M S Cho; H Yee; C Brown; K T Jeang; S Chan
Journal:  Cytotechnology       Date:  2001-09       Impact factor: 2.058
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.