Literature DB >> 8396047

Purification of the 260 kDa cytosolic complex involved in the superoxide production of guinea pig neutrophils.

A Someya1, I Nagaoka, T Yamashita.   

Abstract

A 260 kDa cytosolic complex (SP-1) was purified from guinea pig neutrophils. SP-1 was composed of 63 kDa, 47 kDa and 39 kDa proteins. The 63 kDa and 47 kDa proteins proved to correspond to human p67phox and p47phox by Western blot analysis, whereas Western blot and amino acid sequence analyses revealed that the 39 kDa protein was a novel protein. The 47 kDa protein was separated from the 63 kDa and 39 kDa proteins by dithiothreitol (DTT)-treatment. On the other hand, the 63 kDa and 39 kDa proteins were not separated with DTT, detergent and ethanol treatment. These results suggest that the 39 kDa protein tightly associates with the 63 kDa protein and may regulate the function of the 63 kDa protein.

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Year:  1993        PMID： 8396047     DOI： 10.1016/0014-5793(93)80276-z

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  16 in total

1.  Structure and ligand recognition of the PB1 domain: a novel protein module binding to the PC motif.

Authors:  H Terasawa; Y Noda; T Ito; H Hatanaka; S Ichikawa; K Ogura; H Sumimoto; F Inagaki
Journal:  EMBO J       Date:  2001-08-01       Impact factor: 11.598

Review 2.  Assembly of the phagocyte NADPH oxidase.

Authors:  William M Nauseef
Journal:  Histochem Cell Biol       Date:  2004-08-04       Impact factor: 4.304

3.  Complement-independent Ab-induced peroxide lysis of platelets requires 12-lipoxygenase and a platelet NADPH oxidase pathway.

Authors:  Michael Nardi; Steven J Feinmark; Liang Hu; Zongdong Li; Simon Karpatkin
Journal:  J Clin Invest       Date:  2004-04       Impact factor: 14.808

4.  Interactions between cytosolic components of the NADPH oxidase: p40phox interacts with both p67phox and p47phox.

Authors:  F B Wientjes; G Panayotou; E Reeves; A W Segal
Journal:  Biochem J       Date:  1996-08-01       Impact factor: 3.857

5.  Spontaneous activation of NADPH oxidase in a cell-free system: unexpected multiple effects of magnesium ion concentrations.

Authors:  A R Cross; R W Erickson; B A Ellis; J T Curnutte
Journal:  Biochem J       Date:  1999-02-15       Impact factor: 3.857

6.  Mechanisms of NADPH oxidase activation: translocation of p40phox, Rac1 and Rac2 from the cytosol to the membranes in human neutrophils lacking p47phox or p67phox.

Authors:  S Dusi; M Donini; F Rossi
Journal:  Biochem J       Date:  1996-03-01       Impact factor: 3.857

7.  The adaptor protein p40(phox) as a positive regulator of the superoxide-producing phagocyte oxidase.

Authors:  Futoshi Kuribayashi; Hiroyuki Nunoi; Kaori Wakamatsu; Shohko Tsunawaki; Kazuki Sato; Takashi Ito; Hideki Sumimoto
Journal:  EMBO J       Date:  2002-12-02       Impact factor: 11.598

Review 8.  The NADPH oxidase complex of phagocytic leukocytes: a biochemical and cytochemical view.

Authors:  J M Robinson; J A Badwey
Journal:  Histochem Cell Biol       Date:  1995-03       Impact factor: 4.304

Review 9.  The NADPH oxidase of professional phagocytes--prototype of the NOX electron transport chain systems.

Authors:  Andrew R Cross; Anthony W Segal
Journal:  Biochim Biophys Acta       Date:  2004-06-28

10.  p40phox, a third cytosolic component of the activation complex of the NADPH oxidase to contain src homology 3 domains.

Authors:  F B Wientjes; J J Hsuan; N F Totty; A W Segal
Journal:  Biochem J       Date:  1993-12-15       Impact factor: 3.857
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