Literature DB >> 8395538

A rapid quantitative method for detecting infectious bursal disease virus using polystyrene latex microspheres.

T Nakamura1, A Kato, Z Lin, M Hiraga, T Nunoya, Y Otaki, S Ueda.   

Abstract

A monoclonal antibody (mAb) to infectious bursal disease virus (IBDV) was bound to polystyrene latex microspheres. The microspheres agglutinated with extracts of bursae and sera from chickens infected with all strains or isolates of IBDV tested. Agglutination appeared within a 10-min reaction time. The assay could detect a 10(3.7) to 10(4.5) mean embryo infective dose (EID50) of the virus in 0.01 ml and the titer of the assay was 10- to 40-times higher than that of the agar gel precipitin test.

Entities:  

Mesh:

Substances:

Year:  1993        PMID: 8395538     DOI: 10.1016/0166-0934(93)90095-9

Source DB:  PubMed          Journal:  J Virol Methods        ISSN: 0166-0934            Impact factor:   2.014


  2 in total

1.  Rapid serological profiling by an immunocomb-based dot-enzyme-linked immunosorbent test for three major poultry diseases.

Authors:  S Manoharan; M Parthiban; T G Prabhakar; G Ravikumar; A Koteeswaran; N D J Chandran; G Rajavelu
Journal:  Vet Res Commun       Date:  2004-05       Impact factor: 2.459

2.  Latex immunoassay for rapid detection of Newcastle disease virus.

Authors:  G Thirumurugan; R Jayakumar; K Kumanan; A T Venugopalan; K Nachimuthu
Journal:  Trop Anim Health Prod       Date:  1997-11       Impact factor: 1.559

  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.