| Literature DB >> 8395202 |
L Tabernero1, N Verdaguer, M Coll, I Fita, G A van der Marel, J H van Boom, A Rich, J Aymamí.
Abstract
The molecular structure of the complex between the minor groove binding drug netropsin and the dodecamer d(CGCAAATTTGCG) has been solved and refined by X-ray diffraction analysis to an R-factor of 19.8% and 2.2-A resolution. The drug lies in the narrow minor groove of the B-DNA fragment, covering five of the six A.T base pairs (from A5.T20 to T9.A16). The long six A.T base pair tract allows the drug to bind in a position that optimizes its contacts with the DNA, establishing hydrogen bonds with O2 of thymines and N3 of adenines. The DNA molecule shows a high propeller twist only at the A6.T19 step of the A-tract. Two three-centered hydrogen bonds are observed in the major groove at half of the A-tract.Entities:
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Year: 1993 PMID： 8395202 DOI： 10.1021/bi00084a004
Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162