Literature DB >> 8394673

Pitfalls of in situ polymerase chain reaction (PCR) using direct incorporation of labelled nucleotides.

J F Sällström1, I Zehbe, M Alemi, E Wilander.   

Abstract

False positivity is reported of in situ PCR reactions in a direct incorporation assay with digoxigenin-labelled dUTP. It is recommended that in situ hybridization with specific labelled probe replaces the direct incorporation method for the detection of in situ PCR amplicon.

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Year:  1993        PMID： 8394673

Source DB:  PubMed          Journal:  Anticancer Res        ISSN： 0250-7005            Impact factor:   2.480


  4 in total

1.  In situ polymerase chain reaction-based localization studies support role of human herpesvirus-8 as the cause of two AIDS-related neoplasms: Kaposi's sarcoma and body cavity lymphoma.

Authors:  K E Foreman; P E Bacon; E D Hsi; B J Nickoloff
Journal:  J Clin Invest       Date:  1997-06-15       Impact factor: 14.808

2.  PCR in situ: aspects which reduce amplification and generate false-positive results.

Authors:  I A Teo; S Shaunak
Journal:  Histochem J       Date:  1995-09

3.  In situ PCR for visualization of microscale distribution of specific genes and gene products in prokaryotic communities.

Authors:  R E Hodson; W A Dustman; R P Garg; M A Moran
Journal:  Appl Environ Microbiol       Date:  1995-11       Impact factor: 4.792

4.  Localisation of abundant and organ-specific genes expressed in Rosa hybrida leaves and flower buds by direct in situ RT-PCR.

Authors:  Agata Jedrzejuk; Heiko Mibus; Margrethe Serek
Journal:  ScientificWorldJournal       Date:  2012-05-01
  4 in total

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