BACKGROUND: To study the electrophoretic RNA patterns of human rotavirus (RV) during three years in Valladolid (Spain). MATERIAL AND METHODS: From october 1986 to october 1989 we have studied the electrophoretic RNA patterns of RV in 104 fecal samples. Stools were first screened by a latex agglutination test (LA) and reactive samples were confirmed by a commercial enzymeimmunoassay (EIA) and electron microscopy (EM) (negative stain). Electropherotyping was made according to conventional methods. RESULTS: 96.2% of the samples studied yield a defined electropherotype. We have found 8 different types of patterns of genomic RV-RNA circulating in these three years. Two of them belonged to short pattern (C and E) and six to long pattern (A, B, D, F, G and H). CONCLUSIONS: 98.0% of the genomic RNA founded were of long pattern, from which pattern A was the most prevalent (72%) during the study, without a seasonal distribution. Pattern B was found in 20% of cases, while the rest were found only occasionally.
BACKGROUND: To study the electrophoretic RNA patterns of human rotavirus (RV) during three years in Valladolid (Spain). MATERIAL AND METHODS: From october 1986 to october 1989 we have studied the electrophoretic RNA patterns of RV in 104 fecal samples. Stools were first screened by a latex agglutination test (LA) and reactive samples were confirmed by a commercial enzymeimmunoassay (EIA) and electron microscopy (EM) (negative stain). Electropherotyping was made according to conventional methods. RESULTS: 96.2% of the samples studied yield a defined electropherotype. We have found 8 different types of patterns of genomic RV-RNA circulating in these three years. Two of them belonged to short pattern (C and E) and six to long pattern (A, B, D, F, G and H). CONCLUSIONS: 98.0% of the genomic RNA founded were of long pattern, from which pattern A was the most prevalent (72%) during the study, without a seasonal distribution. Pattern B was found in 20% of cases, while the rest were found only occasionally.
Authors: Francisco Javier Luquero Alcalde; José María Eiros Bouza; Alberto Pérez Rubio; Maria Rosario Bachiller Luque; José Javier Castrodeza Sanz; Raúl Ortiz de Lejarazu Leonardo Journal: Eur J Pediatr Date: 2007-07-25 Impact factor: 3.183