Literature DB >> 8392858

The effect of tissue decalcification on mRNA retention within bone for in-situ hybridization studies.

L Walsh1, A J Freemont, J A Hoyland.   

Abstract

Tissue decalcification is a routine part of the preparation of bone tissue for histological studies. Although in-situ hybridization has been employed to localize mRNA of collagenous and non-collagenous bone related proteins in skeletal tissue, little is known regarding the effects of decalcifying agents on mRNA retention within tissue. In this study in-situ hybridization using an oligonucleotide probe (i.e. a poly d(T) probe) to detect total messenger RNA has been employed to investigate the effects of the decalcifying agents nitric acid, formic acid and EDTA on mRNA retention compared to undeacalcified tissue. The results show that formalin fixation and EDTA decalcification preserve substantial amounts of mRNA within the tissue. In particular, this study illustrates that it is possible to perform in-situ hybridization on formalin fixed decalcified paraffin embedded tissue.

Entities:  

Mesh：

Substances：

Year:  1993        PMID： 8392858      PMCID： PMC2002158     

Source DB:  PubMed          Journal:  Int J Exp Pathol        ISSN： 0959-9673            Impact factor:   1.925


  11 in total

1.  A simple method of defining osteoid in undecalcified sections.

Authors:  J BALL
Journal:  J Clin Pathol       Date:  1957-08       Impact factor: 3.411

2.  Distribution of type X collagen mRNA in normal and osteoarthritic human cartilage.

Authors:  J A Hoyland; J T Thomas; R Donn; A Marriott; S Ayad; R P Boot-Handford; M E Grant; A J Freemont
Journal:  Bone Miner       Date:  1991-11

3.  In situ hybridization demonstration of poly-adenylated RNA sequences in formalin-fixed paraffin sections using a biotinylated oligonucleotide poly d(T) probe.

Authors:  J H Pringle; L Primrose; C N Kind; I C Talbot; I Lauder
Journal:  J Pathol       Date:  1989-08       Impact factor: 7.996

4.  Expression of collagen type transcripts in chick embryonic bone detected by in situ cDNA-mRNA hybridization.

Authors:  S A McDonald; R S Tuan
Journal:  Dev Biol       Date:  1989-05       Impact factor: 3.582

5.  Localization of the expression of types I, III, and IV collagen, TGF-beta 1 and c-fos genes in developing human calvarial bones.

Authors:  M Sandberg; H Autio-Harmainen; E Vuorio
Journal:  Dev Biol       Date:  1988-11       Impact factor: 3.582

6.  Increased production of oxygen free radicals in cigarette smokers.

Authors:  J Kalra; A K Chaudhary; K Prasad
Journal:  Int J Exp Pathol       Date:  1991-02       Impact factor: 1.925

7.  In situ hybridization histochemistry with synthetic oligonucleotides: strategies and methods.

Authors:  M E Lewis; T G Sherman; S J Watson
Journal:  Peptides       Date:  1985       Impact factor: 3.750

8.  Quantitative analysis of in situ hybridization methods for the detection of actin gene expression.

Authors:  J B Lawrence; R H Singer
Journal:  Nucleic Acids Res       Date:  1985-03-11       Impact factor: 16.971

9.  Developmental expression of 2ar (osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridization.

Authors:  S Nomura; A J Wills; D R Edwards; J K Heath; B L Hogan
Journal:  J Cell Biol       Date:  1988-02       Impact factor: 10.539

10.  Localization of types I, II, and III collagen mRNAs in developing human skeletal tissues by in situ hybridization.

Authors:  M Sandberg; E Vuorio
Journal:  J Cell Biol       Date:  1987-04       Impact factor: 10.539
View more
  12 in total

1.  Quantification of mRNA levels in joint capsule and articular cartilage of the murine knee joint by RT-PCR: kinetics of stromelysin and IL-1 mRNA levels during arthritis.

Authors:  J B Van Meurs; P L Van Lent; L A Joosten; P M Van der Kraan; W B Van den Berg
Journal:  Rheumatol Int       Date:  1997       Impact factor: 2.631

2.  Gene expression of matrix metalloproteinases 1, 3, and 9 by chondrocytes in osteoarthritic human knee articular cartilage is zone and grade specific.

Authors:  A J Freemont; V Hampson; R Tilman; P Goupille; Y Taiwo; J A Hoyland
Journal:  Ann Rheum Dis       Date:  1997-09       Impact factor: 19.103

3.  Expression and localization of sulphated glycoprotein-2 mRNA in the rat incisor tooth ameloblasts: relationships with apoptosis.

Authors:  B K Joseph; G C Gobé; N W Savage; W G Young
Journal:  Int J Exp Pathol       Date:  1994-10       Impact factor: 1.925

4.  In situ zymographic localisation of type II collagen degrading activity in osteoarthritic human articular cartilage.

Authors:  A J Freemont; R J Byers; Y O Taiwo; J A Hoyland
Journal:  Ann Rheum Dis       Date:  1999-06       Impact factor: 19.103

5.  Development of an in vitro model to test the efficacy of novel therapies for IVD degeneration.

Authors:  Christine L Le Maitre; Andrew P Fotheringham; Anthony J Freemont; Judith A Hoyland
Journal:  J Tissue Eng Regen Med       Date:  2009-08       Impact factor: 3.963

6.  An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue.

Authors:  Christine L Le Maitre; Pauline Baird; Anthony J Freemont; Judith A Hoyland
Journal:  Arthritis Res Ther       Date:  2009-02-11       Impact factor: 5.156

7.  Performances of single tube nested polymerase chain reaction and GeneXpert ultra on Formalin fixed paraffin embedded tissues in the diagnosis of tuberculous spondylodiscitis.

Authors:  Emna Romdhane; Soumaya Rammeh; Chelli Mouna Bouaziz; Hend Riahi; Meriam Rekaya Ben; Meriam Ksentini; Yosra Chebbi; Wafa Achour; Asma Ferjani; Ben Boubaker Ilhem Boutiba; Leila Slim-Saidi; Mohamed Fethi Ladeb
Journal:  Clin Rheumatol       Date:  2021-06-07       Impact factor: 2.980

8.  The role of interleukin-1 in the pathogenesis of human intervertebral disc degeneration.

Authors:  Christine Lyn Le Maitre; Anthony J Freemont; Judith Alison Hoyland
Journal:  Arthritis Res Ther       Date:  2005-04-01       Impact factor: 5.156

9.  MET expression during prostate cancer progression.

Authors:  Esther I Verhoef; Kimberley Kolijn; Maria J De Herdt; Berdine van der Steen; A Marije Hoogland; Hein F B M Sleddens; Leendert H J Looijenga; Geert J L H van Leenders
Journal:  Oncotarget       Date:  2016-05-24

10.  Possible involvement of the oxLDL/LOX-1 system in the pathogenesis and progression of human intervertebral disc degeneration or herniation.

Authors:  Xinhua Li; Xuejun Wang; Zhouyang Hu; Zhaoxiong Chen; Haoxi Li; Xiaoming Liu; Zhi Yao Yong; Shanjing Wang; Zhanying Wei; Yingchao Han; Jun Tan; Cong Li; Xiao Bo He; Guixin Sun; Desheng Wu; Lijun Li
Journal:  Sci Rep       Date:  2017-08-07       Impact factor: 4.379
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.