High salt alone does not influence the kinetics of the Na(+)-H+ antiporter.

During a high-salt diet, tubular sodium reabsorption is decreased. This study concerns the effect of a high-salt diet on the proximal tubular (PT) Na+ influx pathways. Brush-border membrane vesicles (BBMV) were prepared from rats on normal-salt (NS) and rats on high-salt (HS) diets. The initial uptake rates of Na+ were the same in NS and HS rats, both in the absence and the presence of 1 mM amiloride. Vmax and Km for the amiloride-sensitive Na+/H+ antiporter were also the same in the NS (Vmax 3.69 +/- 0.31 nmol mg prot-1 10 s-1, Km 6.13 +/- 0.58 mM) and HS groups (Vmax 3.54 +/- 0.28 nmol mg prot-1 10 s-1, Km 6.18 +/- 0.64 mM). There was no difference in the initial uptake rates of the Na(+)-glucose and the Na(+)-alanine symporters in NS and HS. Vmax and Km for the L-dopa-Na+ symporter were also the same in NS (Vmax 72 +/- 2.5 pmol mg prot-1 20 s-1, Km 98 +/- 14 microM) and HS groups (Vmax 78 +/- 6.0 pmol mg prot-1 20 s-1, Km 106 +/- 4 microM). In summary, HS diet does not change the kinetics of the Na+ transporters in the brush-border membrane of PT cells.