Metabolic and morphological changes in A. albopictus cells infected with Mayaro virus under heat-shock conditions.

We addressed the question how temperature elevation inhibits Mayaro virus replication in Aedes albopictus infected cells. The morphology and macromolecular changes induced by temperature, infection and high serum concentration were investigated in these cells. Cells incubated with 2 and 10% serum at 28 degrees C disclosed an intense vacuolization and inhibition of [35S]methionine incorporation in a time-dependent manner. 34 and 50 kDa viral structural proteins were detected 24 h after infection. In contrast, an inhibition of viral proteins synthesis occurred when infected cells were kept at 37 degrees C (heat-shock conditions). Total cellular RNA was isolated from mock and infected cells incubated at 28 or 37 degrees C. Northern blot analysis with a Mayaro genomic probe coding for viral structural proteins showed a decrease in the amount of viral 26S RNA in stressed cells when compared to those kept at 28 degrees C. Taken together, these results suggest that the inhibition of viral proteins synthesis in response to temperature elevation is associated with a decrease in the amount of subgenomic 26S RNA.